山东医药2016，Vol.56Issue(40)：25-28,4.DOI:10.3969/j.issn.1002-266X.2016.40.007
环丙沙星对豚鼠心电图和乳头肌动作电位的影响
Effects of ciprofloxacin on QT interval of electrocardiogram and cardiac action potential in papillary muscles of guinea pigs
摘要
Abstract
Objective To observe the effects of ciprofloxacin on electrocardiogram ( ECG) and action potential in papil-lary muscles of guinea pigs, and to investigate the cardio toxicity of ciprofloxacin in order to provide a reference for the safety of clinical application.Methods A total of 42 guinea pigs were randomly divided into 7 groups, with 6 guinea pigs in each group.50, 100, and 200 mg/kg sparfloxacin were injected intraperitoneally into guinea pigs with the low, medium and high dose groups of sparfloxacin, while the low, middle and high dose ciprofloxacin groups were intraperitoneally injected with 50, 100, and 200 mg/kg ciprofloxacin.The control group was injected intraperitoneally with equal doses of NS.Ⅱlead ECG were recorded before and after 5, 10, and 30 minutes of intraperitoneal injection by RM-6240 software.The parameters of ECG were measured, including heart rate, P wave height, PR interval, R wave height, QRS wave width, QT interval and T wave height.A total of 18 guinea pigs were cut off the right ventricular papillary muscle and randomly divided into 3 groups, with 6 guinea pigs in each group.The control group was treated with Tyrode′s solution at constant temperature and constant speed. Sparfloxacin was added to the Tyrode′s solution in the sparfloxacin group, and the concentration of sparfloxacin was increased to 1, 10 and 100μmol/L, respectively.Ciprofloxacin was added to the Tyrode′s solution in ciprofloxacin group, and the final concentration of drug was 1, 10 and 100μmol/L respectively.The time of resting potential (RP), amplitude of action poten-tial ( APA) , action potential duration of 50%repolarization ( APD50 ) , and action potential duration of 90% repolarization (APD90) of guinea pigs′papillary muscles were recorded by RM-6240 software after adding drugs in 15 min.Results At 10 and 30 min after injecting 100 mg/kg sparfloxacin, the QT interval of ECG were prolonged significantly, as compared with the data before injection and that of the control group (all P<0.01).After injecting 200 mg/kg sparfloxacin 5, 10, and 30 min, the QT interval of ECG were prolonged significantly, as compared with the data before injection and the control group (all P<0.01).After injecting 200 mg/kg ciprofloxacin 10, and 30 min, the QT interval of ECG were prolonged significantly as compared with the data before injection and that of the control group (all P<0.05).Ciprofloxacin of medium and high dose groups had a lighter prolonged effect on QT interval than sparfloxacin of medium and high dose groups ( P<0.05 or P<0.01).When the concentration of sparfloxacin in perfusate were 1, 10, and 100 μmol/L, the APD50 and APD90 were pro-longed significantly as compared with that of the control group (all P<0.01).When the concentration of ciprofloxacin in per-fusate was 100μmol/L, the APD90 was prolonged as compared with that of the control group (P<0.01).The effect of 100μmol/L ciprofloxacin in prolonging APD50 as well as APD90 was less than that of 100 μmol/L sparfloxacin (all P<0.01). Conclusions When ciprofloxacin is in high dose, it can cause action potential duration prolong in papillary muscles of guinea pigs, as well as elongate QT interval of ECG in guinea pigs, with potential cardiac toxicity.关键词
环丙沙星/QT间期/乳头肌/动作电位/豚鼠Key words
ciprofloxacin/QT interval/papillary muscles/action potential/guinea pigs分类
医药卫生引用本文复制引用
刘芳,李宜培,师瑞红,陈文超..环丙沙星对豚鼠心电图和乳头肌动作电位的影响[J].山东医药,2016,56(40):25-28,4.基金项目
河南省基础与前沿技术研究计划项目（142300410469）。 （）
