实用医学杂志2016，Vol.32Issue(17)：2807-2812,6.DOI:10.3969/j.issn.1006-5725.2016.17.009
慢病毒干扰TNF-α对脊髓损伤大鼠Caspase-3和Bcl-2表达的影响
Lentivirus-mediated inhibition of tumor necrosis factor-alpha effect in expression of Caspase-3 and Bcl-2 in spinal cord contusion rats
摘要
Abstract
Objective To explore the change of apoptosis factor Caspase-3 and Bcl-2 in the injured segment of rat with spinal cord injury after inhibiting lentivirus expression of inflammation factor TNF-α. To study the relationship between Caspase-3, Bcl-2, Bax and TNF-α in spinal cord injury. Mthods Spinal cord contusion model was prepared by Allen method. The relation between tumor necrosis factor alpha and Bcl-2, was predicted by the method of GeneMANIA bioinformatics. The RNA which was packaged by lentivirus constructed the RNA interference model of tumour necrosis factor alpha. After interference of tumor necrosis factor alpha, we used the method of QRT-PCR to assays the mRNA expression of Caspase-3 and Bcl-2 in spinal cord and detect of the localization of Caspase-3 and Bcl-2 by immunohistochemistry. Statistical analysis with SPSS17.0. Results SD rats had paraplegia and urinate retentaion because of spinal cord injury. The result of QRT-PCR showed that in the seventh day after SCC, the expression of Caspase-3 reduced significantly (P < 0.05) and Bcl-2 did not change significantly (P > 0.05). Immunohistochemistry experiment results showed that Caspase-3 Bcl-2 and Bax immunoreactive cells were observed in the neurons and glial cells of both white matter and gray matter in the spinal cord. The results were the same with QRT-PCR.. Conclusion TNF-α in rats after SCC can effectively regulate the ratio of Bcl-2 and Bax , and then regulate the expression of Caspase-3 , which may affect the function of apoptosis and function recovery after spinal cord injury.关键词
脊髓损伤/TNF-α/Bcl-2/Bax/Caspase-3Key words
Spinal cord injury/TNF-α/Bcl-2/Caspase-3引用本文复制引用
王一枝,胡析,周秀雅,向虹雨,林娅,唐冰怡,唐婷婷,曾茜,张晓..慢病毒干扰TNF-α对脊髓损伤大鼠Caspase-3和Bcl-2表达的影响[J].实用医学杂志,2016,32(17):2807-2812,6.基金项目
成都医学院创新实验项目 （）
