实用医学杂志2016，Vol.32Issue(19)：3160-3164,5.DOI:10.3969/j.issn.1006-5725.2016.19.013
NT-3转染骨髓间充质干细胞对脊髓缺血再灌注的机制研究
The role of bone marrow mesenchymal stem cells transfected with neurotrophin-3 in treatment of spinal cord ischemia-reperfusion injury
摘要
Abstract
Objective To observe the mechanisms of bone marrow mesenchymal stem cells (BMSCs) transfected with neurotrophin-3 in treating nanoparticle repaired spinal cord ischemia-reperfusion (I/R) injury of adult rat. Methods BMSCs were transfected into NT-3 with nanoparticle carrier and transplanted into 80 adult rats on the spinal cord I/R injury model. Then the rats were divided into four groups: sham group, NS group, BMSCs and BMSCs+NT-3. The BBB scoring system, the neurons cell apoptosis, levels of malondialdehyde (MDA) and myeloperoxidase (MPO) were observed and compared between the four groups. Results The BBB score of the BMSCs+NT-3 group was significantly higher than those of BMSCs and NS group , but was lower obviously than the Sham group. TUNEL-apoptosis cells were significantly decreased in the BMSCs + NT-3 group compared to BMSCs and NS groups, but was more significantly lower than the Sham group. The contents of MDA and MPO of the NS group were significantly higher than the BMSCs group. The contents of the BMSCs + NT-3 group were lower than those of the BMSCs group, but lower significantly than the Sham group (P < 0.05). Conclusion BMSCs transfected with NT-3 with nanoparticle carrier could be induced each other. When transplanted into SCI , they can repair spinal cord by alleviating neuron cells apoptosis and inhibiting the lipid peroxidation of free radicals.关键词
神经营养素-3/骨髓间充质干细胞/神经元凋亡/脊髓缺血再灌注损伤/机制Key words
Neurotrophin-3/Bone mesenchymal stem cells/Neuron cells apoptosis/Spinal cord ischemia-reperfusion injury引用本文复制引用
宗海斌,董玉珍..NT-3转染骨髓间充质干细胞对脊髓缺血再灌注的机制研究[J].实用医学杂志,2016,32(19):3160-3164,5.基金项目
河南省医学科技攻关计划项目课题 （）
