实用医学杂志2016，Vol.32Issue(20)：3338-3341,4.DOI:10.3969/j.issn.1006-5725.2016.20.016
MAWBP在结直肠癌细胞增殖侵袭迁移中的作用及分子机制
Molecular mechanism of MAWBP in the proliferation, invasion and migration of colorectal cancer cells
摘要
Abstract
Objective To study the effects and molecular mechanism of MAWBP in the proliferation, invasion and migration of human colorectal cancer cell lines. Methods Colorectal cancer cell lines, caco-2 and HT-29, were divided into MAWBP over-expressed group and control group. MAWBP was stablely over-expressed in both cell lines by lentivirus, and the positive clones were screened by puromycin. The expression of MAWBP gene at mRNA and protein levels was evaluated by RT-PCR and western blot. Cell proliferation was detected by MTT. Cell migration and invasion were detected by transwell experiment. The EMT related protein levels of Snail, E-cadherin, and MAPK pathway protein of ERK, p38, p-ERK, p-p38 were determined by western blot analysis. Results Compared with the control group, the expression of MAWBP was significantly higher in HT-29-MAWBP and caco2-MAWBP group . The cell proliferation , invasion and migration ability significantly reduced in the HT-29-MAWBP group. The expression of Snail, p-ERK and p-p38 significantly decreased, while the E-cadherin increased in HT-29-MAWBP and caco2-MAWBP group. Conclusions Over- expression of MAWBP exerts an inhibitory effect on the proliferation, invasion and migration of colorectal caner cells via inhibit the MAPK pathway and regulate the EMT process.关键词
结直肠癌/MAWBP/增殖/侵袭/迁移Key words
Colorectal cancer/MAWBP/Proliferation/Invasion/migration引用本文复制引用
陈羽青,赵芯梅,李志军,陈声波,刘逸,智发朝..MAWBP在结直肠癌细胞增殖侵袭迁移中的作用及分子机制[J].实用医学杂志,2016,32(20):3338-3341,4.基金项目
国家自然科学基金面上项目 （）
