实用医学杂志2016，Vol.32Issue(20)：3346-3350,5.DOI:10.3969/j.issn.1006-5725.2016.20.018
平阳霉素联合地塞米松对HUVEC细胞增殖和凋亡的影响
Effect of Pingyangmycinand dexamethasone on proliferation and apoptosis of human umbilical vein endothelial cells
摘要
Abstract
Objective To investigate the effect of cell proliferation and apoptosis induced by Pingyangmycin (PYM)and dexamethasone (DEX) on human umbilical vein endothelial cells (HUVEC)in vitro, so as to provide therotical evidence for treatment of aneurysm with PYMand DEX. Methods Control, PYM, DEX and PYM group were established after HUVEC were cultured for 24 hours. Cell morphology was observed by inverted microscope.The effect of cell proliferation and apoptosis were detected with CCK-8reagents and flow Cytometry. The apoptotic protein expression of caspase-3 was testedthrough Western blot. Results Descend of adherent cell density and the ascend of floating cells could be observed after treated with PYM and DEX for 24 hours. HUVEC could be inhibited effectively with concentration-dependent on PYM and DEX. The significant statistical difference of cell apoptosis rate between the group used for PYM alone and the group combined low-concentration PYM with DEX through Flow Cytometrywas found. There was significant statistical difference of apoptotic protein expression of caspase-3 through Western blot compared with the group used for PYM alone and the group combined low-concentration PYM with DEX. Conclusion PYM and DEXcould inhibitthe proliferation of HUVEC alone. The better effects could be observed combination low-concentration PYM with DEX , the mechanism of which might beapoptosis with low-concentration PYM and necrosis with high-concentration PYM.关键词
平阳霉素/地塞米松/血管瘤/细胞增殖/细胞凋亡Key words
Pingyangmycin/Dexamethason/Angeioma/Cell proliferation/Cell apoptosis引用本文复制引用
佟笑,黄益灯,夏思文,康宗辉,卓阳,胡献惠..平阳霉素联合地塞米松对HUVEC细胞增殖和凋亡的影响[J].实用医学杂志,2016,32(20):3346-3350,5.基金项目
“全军十二五”面上项目（编号CWS1IJ256）；南京军区重大专项课题基金资助项目 （）
