实用医学杂志2016，Vol.32Issue(20)：3357-3360,4.DOI:10.3969/j.issn.1006-5725.2016.20.021
大鼠胃窦黏膜AMP-18 mRNA表达减少与胃癌分化程度的关系
Relationship between reduced expressions of AMP-18 mRNA and differentiation degree of gastric cancer
摘要
Abstract
Objective To explore the relationship between the decreased expression of AMP-18 mRNA and different degree of gastric cancer in rats. Methods A total of 20 healthy SD ratswere divided into 4 groups:groups A, B, C and D. A rat model of stomach cancer was established by feeding water with N-Methyl-N′-Nitro-Nitrosoguanidine (MNNG), Tween 20 and vitamin D3. The rats in the four groups were killed respectively at weeks 14, 18, 22 and 24. Then gastric antrum mucosa tissues were detected by using HE staining to observe the pathological changes and by real-time quantitative PCR (Q-PCR) technique to detect the expression of AMP-18 mRNA in rats. Results HE staining section showed that group C developed early gastric cancer, and group D developed progressive gastric cancer. The expression of AMP-18 mRNA in groups C and D wassignificantly lower than that in group A by Q-PCR. Conclusion AMP-18 mRNA expression decreased obviously in early orprogressive gastric cancer.关键词
AMP-18/胃癌分化程度Key words
AMP-18/Differentiated degree of gastric cancer引用本文复制引用
李冰,张珮瑾,穆巴拉克·热和甫,邓广斐,刘英华..大鼠胃窦黏膜AMP-18 mRNA表达减少与胃癌分化程度的关系[J].实用医学杂志,2016,32(20):3357-3360,4.基金项目
广东省大学生创新创业训练计划项目（编号2015105700049）；广州医科大学大学生科技创新项目 （）
