实用医学杂志2016，Vol.32Issue(20)：3364-3368,5.DOI:10.3969/j.issn.1006-5725.2016.20.023
丙泊酚对兔脊髓缺血再灌注损伤血脊髓屏障的保护作用
The protection by Propofol for injured blood-spinal cord barrier in spinal cord ischemia reperfusion in rabbits
摘要
Abstract
Objective To investigate the effects of Propofol in blood spinal cord barrier (BSCB) disruption induced by spinal cord ischemia reperfusion injury (SCIRI). Methods 72 Japanese white rabbits were randomly assigned into 3 groups: sham-operation group (S); ischemia/reperfusion group (I/R) and Propofol treatment group (I/R + P). The Group S was separated the aorta without cross-clamping. SCIRI was induced in rabbits by infrarenal aortic occlusion for 30 minutes. Propofol was intravenously infused at 10 minutes before aortic clamping and at onset of reperfusion in the Group I/R + P. The Group S and Group I/R were intravenously infused 0.9%sodium chloride. Hind-limb motor function was assessed using Tarlov criteria, and histological observation by histological examination. The permeability of the BSCB was examined using EB as vascular tracers. The expression of MMP-9, claudin-5 and NF-κB were assessed by Western blot, RT-PCR. Results Propofol minimized the neuromotor dysfunction and histopathological deficits and attenuated EB extravasation. In addition, Propofol suppressed SCIRI-induced increase of MMP-9 and NF-κB. Finally, Propofol reduced the loss of claudin-5. Conclusion Propofol stabilizes the BSCB integrity after SCIRI. This beneficial effect is partly mediated by inhibition of MMP-9 and preservation claudin-5 and relates to inhibiting the NF-κB signal pathway.关键词
再灌注损伤/脊髓缺血/丙泊酚/血脊髓屏障Key words
Reperfusion injury/Spinal cord ischemia/Propofol/Blood-spinal cord barrier引用本文复制引用
解立杰,黄锦秀,胡霁..丙泊酚对兔脊髓缺血再灌注损伤血脊髓屏障的保护作用[J].实用医学杂志,2016,32(20):3364-3368,5.基金项目
湖北省自然科学基金项目（编号2013CFB086）；中央高校基本科研业务费专项资金资助项目 （）
