天津工业大学学报2016，Vol.35Issue(5)：5-10,6.DOI:10.3969/j.issn.1671-024x.2016.05.002
PP纤维支撑蛋白质印迹海藻酸钙凝胶膜的制备和表征
Preparation and characterization of PP non-woven fiber supported protein molecular imprinted calcium alginate hydrogel film
摘要
Abstract
Bovine serum albumin (BSA) imprinted calcium alginate hydrogel film supported by polypropylene (PP) non-woven fabrics (PP-s-CaAlg MIP) was prepared using sodium alginate as the molecularly imprinted materials, BSA as the template molecule, and PP non-woven fabrics as the matrix. At the same time the non-molecularly imprinted film was also prepared without BSA and named as PP-s-CaAlg NIP. The PP-s-CaAlg MIP was char-acterized by SEM and FT-IR, and its swelling performance was measured in physiological saline. The release properties of BSA on the PP-s-CaAlg hydrogel films prepared with different NaAlg concentrations were investi-gated in different buffers. The adsorption properties of the PP-s-CaAlg MIP and NIP films prepared with different NaAlg concentrations under different buffers were analyzed. The experimental results showed that the PP-s-CaAlg MIP film adsorbed more BSA than PP-s-CaAlg NIP film. Compared with contrast protein, PP-s-CaAlg MIP adsorbed more template protein, indicating that the PP-s-CaAlg MIP exhibited the performance of adsorp-tion selectivity. PP-s-CaAlg MIP exhibited good biocompatibility and could be prepared by a fast , low-cost method, which would find its potential applications in the fields of cell culture and tissue engineering.关键词
蛋白质分子印迹/海藻酸钙凝胶膜/聚丙烯无纺布/释放/吸附Key words
proteins molecularly imprinting/calcium alginate hydrogel film/polypropylene non-woven fabric/release/adsorption分类
轻工纺织引用本文复制引用
宋云飞,魏梦梦,陈甜,焦蕊,孙平平,赵孔银..PP纤维支撑蛋白质印迹海藻酸钙凝胶膜的制备和表征[J].天津工业大学学报,2016,35(5):5-10,6.基金项目
国家自然科学基金资助项目（51103102）；天津市应用基础与前沿技术研究计划项目（16JCZDJC37500）；天津市科技特派员项目 （）
