现代妇产科进展2016，Vol.25Issue(10)：726-729,4.DOI:10.13283/j.cnki.xdfckjz.2016.10.031
miR-101诱导上皮性卵巢癌细胞SKOV3凋亡及其与细胞内Ca2+水平关系的研究
Study on miR-101 induced apoptosis and its relationship to intracellular Ca2+level in epi-thelial ovarian cancer SKOV3 cells
摘要
Abstract
Objective:To study the effect of microRNA-101 ( miR-101 ) on the apopto-sis of epithelial ovarian cancer cell line SKOV3 and to investigate the relationship between the apoptosis and the intracellular Ca2+ concentration. Methods:miR-101 mimics was transfected into SKOV3 cells by using cationic liposomes as a carrierular, and was randomly divided into the control group,the miR-101 negative control group ( miR-101 NC group) and the miR-101 group. The expression level of miR-101 in the three groups was measured by RT-PCR after transfection. The effects of miR-101 on SKOV3 cells apoptosis and on the concentration of intra-cellular Ca2+were evaluated by flow cytometry. Also,the expression of caspase-3 protein was an-alyzed by Western blot. Results:RT-PCR results revealed that the expression of miR-101 in miR-101 group (5. 302±0. 069) was increased by 5. 10 fold and 4. 70 fold respectively com-pared with the control group ( 1 . 038 ± 0 . 036 ) and the miR-101 NC group ( 1 . 126 ± 0 . 062 ) , with statistical correlations of F=5326 . 276 and P=0 . 000 . The flow cytometry suggested that the percentage of apoptosis cells and the concentration of intracellular calcium was increased significantly in the miR-101 group [ ( 66 . 36 ± 13 . 54 )%, ( 228 . 94 ± 19 . 51 ) nmol/L ] compared with the control group[(40. 60±5. 97)%,(62. 91±7. 73)nmol/L]and the miR-101 NC group [(41. 54±3. 89)%,(59. 09±10. 66)nmol/L](P=0. 019). In addition,Western blot analysis suggested that caspase-3 expression in the miR-101 group ( 1 . 325 ± 0 . 052 ) was up-regulated compared with the control group (0. 817±0. 073) and the miR-101 NC group (0. 820±0. 062) ( P=0 . 000 ) . Conclusions:Overexpression of miR-101 could induce apoptosis of epithelial o-varian cancer SKOV3 cells,and the induction of apoptpsis may be related to the increase of in-tracellular Ca2+ level.关键词
卵巢癌/miR-101/细胞凋亡/细胞内Ca2+水平/Caspase-3Key words
Ovarian cancer/miR-101/Apoptosis/Intracellular Ca2+ level/Caspase-3分类
医药卫生引用本文复制引用
王秀娟,韩萍,曹燕花,代娟,杨莉,于笑涵..miR-101诱导上皮性卵巢癌细胞SKOV3凋亡及其与细胞内Ca2+水平关系的研究[J].现代妇产科进展,2016,25(10):726-729,4.基金项目
河北省临床医学优秀人才培养(No:310103020704) （No:310103020704）
