现代妇产科进展2016，Vol.25Issue(10)：730-733,4.DOI:10.13283/j.cnki.xdfckjz.2016.10.002
卵巢癌大网膜转移差异基因的筛选及验证
Preliminary screening and identification of differentially expressed metastasis-related genes in ovarian cancer
摘要
Abstract
Objective:To find out metastasis-related genes in ovarian cancer though preliminary screenning and identificating of the diferentially expressed genes in primary ovarian cancer and omentum metastasis samples. Methods:Microarray analysis was performed to uncov-er genes that were differentially expressed between three pair of matched primary ovarian cancer and omental metastasis. QuantitativeReal-time PCR ( qRT-PCR ) and immunohistochemistry ( IHC) were used to validate the microarray results at the mRNA and protein levels,respective-ly. Result:Microarray revealed that 187 mRNAs were differentially expressed. Among which 160 genes were up-regulated and 27 were down-regulated in omental metastasis. Among these blotted genes,the markedly differentially expression of Interleukin 8(IL-8) and armadillo repeat containing 9(ARMC9)were preliminary screenned. Fourthermore,the expressions of IL-8 and ARMC9 in omentum metastasis were identified both significantly higher than that of ovarian cancer by qRT-PCR and IHC ( P<0 . 05 ) . Conclusion:IL-8 and ARMC9 were related to the progress of ovarian cancer metastasize.关键词
卵巢癌/大网膜转移/基因芯片/Interleukin 8/ARMC9Key words
Ovarian cancer/Omentum metastasis/Microarray/Interleukin 8/ARMC9分类
医药卫生引用本文复制引用
张淑芬,谢娅姣,朱君佑,付琨,杨宇,张瑜..卵巢癌大网膜转移差异基因的筛选及验证[J].现代妇产科进展,2016,25(10):730-733,4.基金项目
中南大学研究生自主探索创新基金项目(No:2015zzts297) （No:2015zzts297）
湖南省科技计划项目(No:2013FJ4114) （No:2013FJ4114）
