现代妇产科进展2016，Vol.25Issue(10)：734-738,743,6.DOI:10.13283/j.cnki.xdfckjz.2016.10.003
Anti-cMet抗体耦合空心纳米金球对宫颈癌光热治疗增敏作用研究
Anti-cMet antibody conjugated hollow gold nanospheres as a new nano-material for en-hancing the effect of photothermal therapy
摘要
Abstract
Objective:To investigate the molecular mechanism of anti-cMet/HGNs in mediating targeted cancer treatment and enhancing treatment sensitivity in photothermal thera-py. Methods:The successful synthesis of HGNs was observed and photographed by transmission electron microscope ( TEM) . The absorbance of naked HGNs and anti-cMet/HGNs was exam-ined by UV-Visible-NIR spectrophotometry. Over-representation of cMet in cancer cells was ver-ified by immunofluorescence. Uptake of HGNs and anti-cMet/HGNs was tested by inductively coupled plasma atomic emission spectroscopy ( ICP-AES ) . The in vitro photothermal therapy was conducted in four groups-control,laser alone,naked HGNs with laser and anti-cMet/HGNs with laser. To test the toxicity of functionalized gold nanospheres using cell counting kit-8(CCK-8). To investigate these four groups' inhibitory rate of cell proliferation by CCK-8. The expres-sion of apoptosis-related proteins was examined by western blot. Result:Modification with PEG and conjunction with targeting antibody made an approximately 20 nm right shift of the largest infrared absorption peak (from 788nm to 808nm) of gold nanomaterials. Both two human cervi-cal cancer cell lines-HeLa and CaSki-were detected a layer of Fluorescent green marker. Corre-spondingly,ICP-AES confirmed the distinct uptake of anti-cMet/HGNs by both two cell lines. IC50 was 1. 1nmol/L in HeLa and 0. 6nmol/L in CaSki. Compared with other three groups,anti-cMet/HGNs with laser (1. 7J/cm2 ) possessed a higher inhibitory rate of cell proliferation by 34. 8% in HeLa and 53. 6% in CaSki. Meanwhile this group was indicated obviously overex-pression of Caspase-3,Bax and Cyclin E2 and a low expression of Bcl-2 in both two cell lines. Conclusions:Anti-cMet/HGNs have the potentials to breaking Bcl-2/Bax balance to induce ap-optosis and to enhancing the effect of photothermal ablation therapy on cervical cancer.关键词
纳米微球/光热治疗/宫颈癌/靶向治疗Key words
Nanospheres/photothermal ablation therapy/cervical cancer/targeting therapy分类
医药卫生引用本文复制引用
梁莹,王丽珍,刘婷,陈召龙,初剑英,杨兴升..Anti-cMet抗体耦合空心纳米金球对宫颈癌光热治疗增敏作用研究[J].现代妇产科进展,2016,25(10):734-738,743,6.基金项目
国家自然科学基金资助项目(No:81372809) （No:81372809）
山东省科技发展计划项目(No:2014GGH218037) （No:2014GGH218037）
