现代妇产科进展2016，Vol.25Issue(10)：744-746,3.DOI:10.13283/j.cnki.xdfckjz.2016.10.005
IDO与卡铂诱导卵巢癌耐药的免疫学研究
Study on IDO and chemotherapy resistance ovarian cancer induced by carboplatin in Immunology
摘要
Abstract
Objective:Study on the mechanism of resistant ovarian cancer metastasis or recurrence from the perspective of immunology by carboplatin induced multidrug resistance in o-varian carcinoma cell line effect on cellular immune function. Methods:The resistant SKOV3/CBP cell lines were established by carboplatin inducing human ovarian cancer SKOV3 cells. In-dole 2,3-dioxygenase ( IDO) expressions of SKOV3 and SKOV3/CBP cells were detected by ELISA method. Then the two groups of cells were cultured with lymphocytes,NK cells and C8+T cells separately,lymphocyte proliferation were monitored by MTT method,the killing ability of NK cell were tested by LDH release assay,the function of C8+T cells were detected by ELIS-POT assay. Results:Compared with SKOV3 cell lines,the expression of IDO was significantly increased in SKOV3/CBP cell lines (P<0. 05),and the proliferation ability of lymphocyte,NK cell killing capacity and CD8+T cell killing ability of SKOV3/CBP cells group were significantly decreased (P<0. 05) than that of SKOV3 cells group. Conclusion:SKOV3/CBP cells that in-creased expression of IDO can reduce the sensitivity of the immune cells and immune escape, promote metastasis and relapse of chemotherapy resistant tumors further. IDO can be new prom-ising targets for treatment of carboplatin resistance of ovarian carcinoma.关键词
卵巢癌/卡铂/耐药性/IDO/免疫功能Key words
Ovarian cancer/Carboplatin/Chemotherapy resistance/IDO/Immune function分类
医药卫生引用本文复制引用
冯勤梅,白宁,李莉,彭梅..IDO与卡铂诱导卵巢癌耐药的免疫学研究[J].现代妇产科进展,2016,25(10):744-746,3.基金项目
山西省自然基金资助项目(No:2013011045-5) （No:2013011045-5）
