摘要
Abstract
Objective:To investigate the expression of Retinol Binding Protein 4 and its correlation with insulin resistance and insulin secretion in placenta of gestational diabetes melli-tus. Methods:79 placental tissues were collected including 27 pregnant women who had normal glucose tolerance test. There are 52 pregnant women with GDM,including 26 cases of GDMA1 , and 26 cases of GDMA2 . The expression of RBP4 in placenta was detected by immunohisto-chemistry(IHC),Western blot and quantitative real-time PCR(qRT-PCR). Insulin resistance and insulin secretion function were measured by homeostasis insulin resistance index ( HOMA-IR) and homeostasisβ-cell index ( HBCI) respectively. The results above were compared among the three groups. The correlation of RBP4 expression with HOMA-IR and HBCI was invested. Results:RBP4 was located on the cytoplasm of syncytiotrophoblast. The expression of RBP4 in GDM groups were higher than that in normal group. The differences between GDM groups had no statistically significant(P>0. 05). From Normal group to GDMA1 group and GDMA2 group,HOMA-IR increased,which in GDM groups were higher than that in normal group (P<0. 01). However,the differences between GDM groups had no statistically significant(P>0. 05). From Normal group to GDMA1 group and GDMA2 group,HBCI increased firstly and then decreased, which in GDMA2 group were lower than that in the another two groups(P<0. 01). However,the differences between Normal and GDMA1 had no statistically significant(P>0. 05). The protein level of RBP4 in placenta of GDM group was positively correlated with HOMA-IR (r=0. 819,P<0 . 01 ) . However there was no correlation with HBCI ( r=0 . 179 , P=0 . 123 ) . There was no correlation between the protein level of RBP4 in placenta of GDM group with HOMA-IR and HBCI. Conclusion:RBP4 is secreted by placenta,causes the occurrence of GDM by attending insulin resistance. However,there is no obvious proof display the relation between RBP4 and in-sulin secretion function.关键词
视黄醇结合蛋白4(RBP4)/妊娠期糖尿病(GDM)/胎盘/胰岛素抵抗/胰岛素分泌功能Key words
Retinol binding protein 4/Gestational diabetes mellitus/Placenta/Insulin resistance/Insulin secretion function分类
医药卫生
