现代妇产科进展2016，Vol.25Issue(10)：757-761,5.DOI:10.13283/j.cnki.xdfckjz.2016.10.009
COX2对卵巢透明细胞癌细胞ES-2顺铂耐药的影响及相关机制
The role cyclooxygenase-2 act in cisplatin chemotherapy of ovarian clear-cell cancer and related mechanisms
摘要
Abstract
Objective:To examine the role COX2 act in ovarian clear-cell cancer cells cisplatin-resistance and its related mechanisms. Methods:We created Lenti-COX2-EGFP and then transfected ovarian cancer cells. We silenced COX2 expression in ES-2 cells. The effect of COX2 on proliferation and drug-resistance effect of ovarian cancer was evaluated using CCK-8 , PCR,Western blot. Results:COX2 overexpression promoted cell proliferation and silence COX2 inhibit cell proliferation. Up-regulated COX2 expression promoted ES-2 cell resistance to cispla-tin and the indx of drug resistance was 1 . 26 . Silenced the expression of COX2 inhibited cispla-tin resistance and the reverse yold was about 6. 68 times. Slienced COX2 expression in ES-2 cells can down-regulate the expressions of Snail,Slug and Vimentin,and up-regulate the expres-sions of E-cadherin. Conclusion:COX2 promotes ES-2 cells resistance to cisplatin, inducing EMT maybe one of its mechanisms.关键词
卵巢透明细胞癌/COX2/耐药/顺铂/EMTKey words
Ovarian clear cell cancer/COX2/Drug-resistance/Cisplatin/EMT分类
医药卫生引用本文复制引用
邓琳,冯定庆,凌斌..COX2对卵巢透明细胞癌细胞ES-2顺铂耐药的影响及相关机制[J].现代妇产科进展,2016,25(10):757-761,5.基金项目
国家自然科学基金资助项目(No:81372777,81372779) （No:81372777,81372779）
