中风与神经疾病杂志2016，Vol.33Issue(10)：894-896,3.
灯盏细辛与赤芍配伍对永久性脑缺血损伤大鼠的保护作用
Compatibility of erigeron breviscapus and radix paeoniae rubra protects rats against cerebral ischemic injury
摘要
Abstract
Objective To investigate the protective effect of the compatibility of Erigeron breviscapus and Radix Paeoniae Rubra on cerebral ischemia injury rats and its possible mechanisms .Methods Forty-eight adult male Sprague-Dawley rats were divided into four groups randomly:a sham operation group ,a cerebral ischemia group ,a treatment group and an inhibitor group,12 in each group.MCAO model was performed according to Zea-longa’s report.Neurological deficit scores were performed after 24 h of the operation .Then decapitated the rats and took out their brain .Detected infarct size by 2,3,5-triphenyltetrazolium chloride (TTC) staining,and detected the expression of the mRNA levels of Shh、Patched-1、Gli-1 by RT-PCR and assessed the proteins expression of them by Western blotting .Results The neurological deficit score and the infarct size in the treatment group were lower than that in the cerebral ischemia and inhibitors groups ,both of the differences were statistically significant .RT-PCR and Western blotting suggested that the mRNA and protein levels of Shh、Patched-1、Gli-1 in the treatment group was higher than that in the cerebral ischemia group .The the GLI-1 level in the inhibitor group was lower than the treatment group .The differences were statistically significant .Conclusion The compatibility of Erigeron breviscapus and Radix Paeoniae Rubra has certain protective effect on permanent cerebral ischemia injury rats .Its mechanism was associ-ated with the up-regulation of the expression of the key components of Sonic Hedgehog signaling pathway .关键词
灯盏细辛/赤芍/脑缺血/配伍Key words
Erigeron breviscapus/Radix Paeoniae Rubra/Cerebral ischemia/Compatibility分类
医药卫生引用本文复制引用
范娅丹,韩江全,邓才洪..灯盏细辛与赤芍配伍对永久性脑缺血损伤大鼠的保护作用[J].中风与神经疾病杂志,2016,33(10):894-896,3.基金项目
贵州省中医药、民族医药课题（黔中医药20141433）；珠海市重点学科项目 （）
