中国药房2016，Vol.27Issue(30)：4257-4260,4.DOI:10.6039/j.issn.1001-0408.2016.30.26
HPLC法同时测定参菊洗剂中4种成分的含量
Simultaneous Determination of Four Components in Shenju Lotion by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of matrine,linarin,berberine hydrochloride and osthole in Shenju lotion. METHODS:HPLC was performed on the column of Dionex C18 with mobile phase of acetonitrle-0.1% triethylamine(pH was adjusted to 7.2 with phosphoric)(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 210 nm,column temperature was 30 ℃,and injection volume was 20 μl. RESULTS:The linear range was 1.400-8.400 μg for matrine (r=0.999 4),0.152-0.912 μg for linarin (r=0.999 6),0.248-1.488 μg for berberine hydrochloride (r=0.999 9) and 0.128-0.768 μg for osthole (r=0.999 4);limit of quantitation was 84.85,3.15,15.03,7.76 ng,limit of detection was 24.13, 0.89,4.27,2.20 ng;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 96.29%-99.06%(RSD=1.10%,n=9),96.49%-98.27%(RSD=0.69%,n=9),99.26%-101.13%(RSD=0.62%,n=9) and 96.27%-98.64%(RSD=0.89%,n=9). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the simultaneous determination of matrine,linarin,berberine hydrochloride and osthole in Shenju lotion.关键词
参菊洗剂/苦参碱/蒙花苷/盐酸小檗碱/蛇床子素/高效液相色谱法/含量测定Key words
Shenju lotion/Matrine/Linarin/Berberine hydrochloride/Osthole/HPLC/Content determination分类
医药卫生引用本文复制引用
谭璐,张广求,刘文..HPLC法同时测定参菊洗剂中4种成分的含量[J].中国药房,2016,27(30):4257-4260,4.基金项目
黄冈市科技计划项目 （）
