中国药房2016，Vol.27Issue(30)：4264-4266,3.DOI:10.6039/j.issn.1001-0408.2016.30.28
RP-HPLC法同时测定半夏糖浆中乙酸和琥珀酸的含量
Simultaneous Determination of Acetic Acid and Succinic Acid in Banxia Syrup by RP-HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of acetic acid and succinic acid in Banxia syrup. METHODS:RP-HPLC was performed on the column of GL InterSustain-C18 with mobile phase of 0.03 mol/L ammonium di-hydrogen phosphate buffer solution(pH2.0)-methanol(gradient elution)at a flow rate of 0.8 ml/min,the detection wavelength was 210 nm,column temperature was 30 ℃,injection volume was 10 μl. RESULTS:The linear range was 0.020 98-0.209 8 μg/ml for acetic acid(0.999 9)and 12.04-120.4 μg/ml for succinic acid(r=0.999 9);limits of quantification were 0.15,18.24 ng,limits of detection were 0.045, 5.53 ng;RSDs of precision, stability and reproducibility tests were lower than 2%;recoveries were 97.45%-101.68%(RSD=1.39%,n=9) and 98.31%-101.08%(RSD=1.01%,n=9). CONCLUSIONS:The method is accurate and rapid,and suitable for the simultaneous determination of acetic acid and succinic acid in Banxia syrup.关键词
反相高效液相色谱法/半夏糖浆/乙酸/琥珀酸Key words
RP-HPLC/Banxia syrup/Acetic acid/Auccinic acid分类
医药卫生引用本文复制引用
何丹,杨晓梅,傅亚,秦少容,张景勍..RP-HPLC法同时测定半夏糖浆中乙酸和琥珀酸的含量[J].中国药房,2016,27(30):4264-4266,3.基金项目
重庆市教委科学技术研究项目 （）
