中国中医药信息杂志2016，Vol.23Issue(11)：57-61,5.DOI:10.3969/j.issn.1005-5304.2016.11.014
丹参通络解毒汤对骨髓干细胞移植急性心肌梗死模型大鼠炎症反应的影响
Effects ofDanshen Tongluo Detoxication Decoction on Inflammatory Response of Rats with Bone Marrow Stem Cell Transplantation in Acute Myocardial Infarction
摘要
Abstract
Objective To explore the effects ofDanshen Tongluo Detoxication Decoction on the inflammatory response of rats with bone marrow stem cell (BMSC) transplantation in acute myocardial infarction (AMI); To discuss its mechanism of action.Methods The whole bone marrow adherent method was adopted for BMSCs separation and culture. The AMI model was established by closing the left anterior descending coronary artery of SD rats. After the modeling, SD rats were randomly divided into sham-operation group, model group and BMSCs group (BMSCs transplantation group),Danshen Tongluo Detoxication Decoction group, Danshen Tongluo Detoxication Decoction + BMSCs group, 10 rats in each group. BMSCs cell suspension was injected directly into the edge of the myocardial tissue infarction area; Chinese medicine or normal saline were administered for gavage. 4 weeks later, the contents of MCP-1 and sICAM-1 in each group were detected by ELISA. TLR-4 expression was measured by Western blot method, and HE staining was used to observe the myocardial tissue pathological changes.Results Compared with the model group, the contents of MCP-1 and sICAM-1 and the protein expression of TLR-4 in BMSCs group,Danshen Tongluo Detoxication Decoction group, andDanshen Tongluo Detoxication Decoction + BMSCs group decreased, Danshen Tongluo Detoxication Decoction group was better than BMSCs group (P<0.05,P<0.01), andDanshen Tongluo Detoxication Decoction + BMSCs group was better than BMSCs group andDanshen Tongluo Detoxication Decoction group(P<0.05,P<0.01).Conclusion BMSCs transplantation combined withDanshen Tongluo Detoxication Decoction can restrain the inflammatory response of AMI model rats and repair ischemic myocardium issue, which mechanism may be related to regulating TLR-4 induced inflammatory response.关键词
丹参通络解毒汤/急性心肌梗死/单核细胞趋化蛋白-1/可溶性细胞间黏附因子-1/Toll 样受体4/炎症反应/大鼠Key words
Danshen Tongluo Detoxication Decoction/acute myocardial infarction/MCP-1/sICAM-1/TLR-4/inflammatory response/rats分类
医药卫生引用本文复制引用
李鑫辉,李雅婧,黄淼鑫,杜建芳,许福丽,肖青,郭晨鹤..丹参通络解毒汤对骨髓干细胞移植急性心肌梗死模型大鼠炎症反应的影响[J].中国中医药信息杂志,2016,23(11):57-61,5.基金项目
湖南省教育厅科学研究重点项目 （）
湖南省中医药科研计划项目（201559）；国家中医药管理局重点学科开放基金 （）
