中国中医药信息杂志2016，Vol.23Issue(11)：62-66,5.DOI:10.3969/j.issn.1005-5304.2016.11.015
香砂六君子汤对脾胃虚弱型萎缩性胃炎大鼠胃窦黏膜组织白细胞介素-6、10及热休克蛋白70表达的影响
Effects of Xiangsha Liujunzi Decoction on Levels and mRNA of IL-6, IL-10 and Protein Expression of HSP70 of Gastric Mucosa in Chronic Atrophic Gastritis Rats with Spleen-Stomach Deficiency
摘要
Abstract
Objective To observe the effects ofXiangsha Liujunzi Decoction (XSLJZ) on levels and mRNA of IL-6, IL-10 and protein expression of HSP70 of gastric mucosa in chronic atrophic gastritis (CAG) rats with spleen-stomach deficiency; To discuss its mechanism.Methods Rats were divided into 2 groups through random number table: normal group and model group. The model of CAG rat with spleen-stomach deficiency type was induced by synthetic methods. After successful modeling, rats were divided into model group, positive control group, XSLJZ high-, medium-, low-dose group. Rats in normal and model group received distilled water 10 mL/(kg•d) for gavage; XSLJZ high-, medium-, low-dose group received XSLJZ 24, 12, 6 g/(kg•d), respectively; positive control group received mycin 0.30 g/(kg•d) for gavage for 120 consecutive days. Generally living conditions, levels and mRNA of IL-6, IL-10 and protein expression of HSP70 in gastric mucosa tissue were detected by protein immunoblotting.Results Compared with the normal group, generally living conditions of rats in the model group were poor; mRNA and the content of IL-6 increased significantly, and mRNA and the content of IL-10 decreased significantly (P<0.05,P<0.01); mRNA and protein expression of HSP70 in gastric tissues was much lower than that of normal group (P<0.05,P<0.01). Compared with model group, generally living conditions of rats in the XSLJZ high-dose groups were improved significantly; mRNA and the content of IL-6 decreased significantly, and mRNA and the content of IL-10 increased significantly (P<0.05); XSLJZ high-, medium-dose groups mRNA and protein expression of HSP70 in gastric tissues increased significantly(P<0.05).Conclusion XSLJZ has protective effects on gastric mucosa of CAG rats with spleen-stomach deficiency.关键词
香砂六君子汤/萎缩性胃炎/白细胞介素-6/白细胞介素-10/热休克蛋白70/大鼠Key words
Xiangsha Liujunzi Decoction/atrophic gastritis/IL-6/IL-10/HSP70/rats分类
医药卫生引用本文复制引用
王强,王庆胜,鲁鹏程,成映霞,李兰珍,段云燕,段永强,杜娟,刘雪松..香砂六君子汤对脾胃虚弱型萎缩性胃炎大鼠胃窦黏膜组织白细胞介素-6、10及热休克蛋白70表达的影响[J].中国中医药信息杂志,2016,23(11):62-66,5.基金项目
国家自然科学基金（81260519）；甘肃省中医药管理局基金项目（GZK-2014-73）；甘肃省中医方药挖掘与创新转化重点实验室开放基金 （）
