中国中医药信息杂志2016，Vol.23Issue(11)：78-80,3.DOI:10.3969/j.issn.1005-5304.2016.11.019
一测多评法与外标法测定胆黄连中生物碱类成分含量比较
Comparison of Simultaneous Determination of Alkaloids in Bile Processed Coptidis Rhizoma by QAMS and External Standard Method
摘要
Abstract
Objective To establish QAMS method to determine the contents of three alkaloids in bile processed Coptidis Rhizoma; To compare the results of QAMS with those from external standard method; To prove the feasibility of QAMS.Methods An HPLC method was developed. Berberine hydrochloride was selected as the internal reference substance. 2 relative correction factors (RCF) of berberine hydrochloride to palmatine hydrochloride and to jatrorrhizine hydrochloride were established. Obtained RCFs were used to conduct content calculation (calculated value) to complete QAMS method. At the same time, the contents (measured value) of the three components were also determined by external standard method. Calculated value and measured value were compared.Results The analysis results showed that there was no significant difference between the calculated values and the measured values of the three alkaloids in 10 batches of bile processed Coptidis Rhizoma.Conclusion The QAMS method can be applied in the determination of alkaloids in bile processed Coptidis Rhizoma.关键词
一测多评法/外标法/胆黄连/生物碱/相对校正因子Key words
QAMS/external standard method/bile processed Coptidis Rhizoma/alkaloids/relative correction factors分类
医药卫生引用本文复制引用
王静,陈悦,袁子民..一测多评法与外标法测定胆黄连中生物碱类成分含量比较[J].中国中医药信息杂志,2016,23(11):78-80,3.基金项目
国家自然科学基金 （）
