中国中医药信息杂志2016,Vol.23Issue(11):103-106,4.DOI:10.3969/j.issn.1005-5304.2016.11.025
不同产地五味子药材色谱图谱研究
Study on HPLC Fingerprints of Schisandrae Chinensis Fructus from Different Producing Areas
摘要
Abstract
Objective To establish the fingerprint analysis method of Schisandrae Chinensis Fructus by HPLC; To analyze the similarity on the fingerprints of Schisandrae Chinensis Fructus from different producing areas. Methods The chromatographic separation was performed by HPLC on a Diamonsil C18 column (250 mm×4.6 mm, 5 µm). Acetonitrile-water was used as gradient mobile phase. The flow rate was 1.0 mL/min. The column temperature was maintained at 30℃. The detection wavelength was set at 254 nm.Results The HPLC fingerprint analysis method of Schisandrae Chinensis Fructus was established. Twenty-nine common fingerprint peaks were identified. The similarities of the fingerprints of ten samples from different producing areas were above 0.95.Conclusion The method is simple and reliable, which can provide a scientific basis for quality evaluation of Schisandrae Chinensis Fructus.关键词
五味子/指纹图谱/高效液相色谱法/质量控制Key words
Schisandrae Chinensis Fructus/fingerprint/HPLC/quality control分类
医药卫生引用本文复制引用
王连芝,付克,顾媛媛,刘磊,张淑香,周琦..不同产地五味子药材色谱图谱研究[J].中国中医药信息杂志,2016,23(11):103-106,4.基金项目
黑龙江省教育厅科学技术研究项目 ()