作物学报2016，Vol.42Issue(11)：1577-1591,15.DOI:10.3724/SP.J.1006.2016.01577
棉花酰基辅酶A结合蛋白（ACBP）家族基因的发掘及在非生物胁迫抗性中的功能鉴定
Genome-wide Identification of Acyl-CoA-Binding Protein (ACBP) Gene Family and Their Functional Analysis in Abiotic Stress Tolerance in Cotton
摘要
Abstract
Acyl-CoA-binding protein (ACBP) plays important roles in plant development, including normal growth, response to biotic/abiotic stress and membrane system repairing. However, the functions ofACBPs in cotton still remain largely unknown. Based on theG. hirsutum TM-1 genome database, we identified 21ACBP genes and obtained their sequence information, chro-mosomal location. Phylogenetic analysis revealed that the 21ACBP genes were classified into four groups. According to the no-menclature ofA. thaliana homologous gene, the cottonACBP genes were named asGhACBP1–GhACBP6 subclasses. Transcrip-tome data showed that the expression patterns ofACBP genes varied significantly in different cotton tissues. Further abiotic stresses treatment analysis showed thatGhACBP1,GhACBP3, andGhACBP6 sub-class genes could be significantly induced by drought, salt, low temperature and high temperature treatments, whileGhACBP4 andGhACBP5 sub-class genes could not. GhACBP3 andGhACBP6 could also be significantly induced by hydrogen peroxide (H2O2), salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA), and ethylene (ET). Virus induced gene silencing (VIGS) results exhibited that down-regulation of GhACBP3 andGhACBP6 sub-class genes would significantly reduce the plant tolerance to drought and salt stresses. Compared with the control, theGhACBP3 andGhACBP6 silencing plants demonstrated significantly reduced plant dry matter, decreased plant height, lower superoxide dismutase (SOD) and peroxidase (POD) activities, and increased malondialdehyde (MDA) content. These results support the important functions ofGhACBP3 andGhACBP6 in cotton tolerance to drought and salt. This study will provide useful information for further use ofACBP genes in future cotton studies and breeding practice.关键词
棉花/ACBP基因家族/结构/表达/干旱胁迫/盐胁迫Key words
Cotton/ACBP gene family/Structure/Expression/Drought stress/Salt stress引用本文复制引用
秦朋飞,尚小光,宋健,郭旺珍..棉花酰基辅酶A结合蛋白（ACBP）家族基因的发掘及在非生物胁迫抗性中的功能鉴定[J].作物学报,2016,42(11):1577-1591,15.基金项目
本研究由国家转基因生物新品种培育重大专项(2015ZX08005-004)和江苏现代作物生产协同创新中心(No.10)项目资助。 This program was financially supported in part by the National Majar Project for Developing New GM Crops (2011ZX08005-004) and Jiangsu Collaborative Innovation Center for Modern Crop Production Project (No.10) （2015ZX08005-004）
