作物学报2016，Vol.42Issue(11)：1620-1628,9.DOI:10.3724/SP.J.1006.2016.01620
大豆四向重组自交系群体蛋白质含量与油分含量QTL定位
Mapping QTL Protein and Oil Contents Using Population from Four-way Re-combinant Inbred Lines for Soybean (Glycine max L. Merr.)
摘要
Abstract
Increasing protein content (PC) and oil content (OC) are main goals in soybean improvement, so mapping quantitative trait locus (QTL) and mining elite alleles underlying PC and OC are of importance for molecular design breeding in soybean. In this research a four-way recombinant inbred line population derived from double cross (Kenf 14 × Kenf 15) × (Hein 48 × Kenf 19) with 204 lines was used to analyze the data of PC and OC from the field experiments in eight environments across Harbin and Keshan in 2013, 2014, and 2015 by interval map method based on a linkage map constructed in previous research. The 29 PC QTLs and 39 OC QTLs were detected from eight planting environments. Among the twenty-nine PC QTLs, five were detected across over two environments, which distributed on six linkage groups, i.e. A1, D2, J, N and O, with explained phenotypic varia-tion (PVE) ranging from 7.65% to 20.08%. Four of them, i.e.qPC-A1-1,qPC-D2-1,qPC-J-1, andqPC-O-2, showed PVE over 10%. Of the thirty-nine OC QTLs, ten were found in more than two environments, which were located on linkage groups A1, A2, B1, D1b, G, I, J, and N with PVE ranging from 7.30% to 25.68%. Four out of the ten QTLs that includedqOC-A2-1,qOC-B1-1, qOC-G-1,andqOC-J-1had PVE above 10%.关键词
大豆/蛋白质含量与油分含量/QTL/四向重组自交系群体/优异等位基因Key words
Soybean/Protein and oil content/QTL/Four-way recombinant inbred line/Elite allele引用本文复制引用
宁海龙,白雪莲,李文滨,薛红,庄煦,李文霞,刘春燕..大豆四向重组自交系群体蛋白质含量与油分含量QTL定位[J].作物学报,2016,42(11):1620-1628,9.基金项目
本研究由?黑龙江省自然科学基金面上项目(C2015007),黑龙江省省留学归国人员科学(LC2016010),黑龙江省教育厅科学技术研究重点项目(12541z001)和黑龙江省博士后科研启动基金(LBH-Q12152, LBH-Q09165)资助。 This study was supported by the Natural Science Foundation of Heilongjiang Province (C2015007), the Study Abroad Returnees Science Fund of Heilongjiang Province (LC2016010), Science and Technology Research Key Project of Heilongjiang Province Department of Educa-tion (12541z001), and Postdoctoral Research Start Fund of Heilongjiang Province (LBH-Q12152, LBH-Q09165) （C2015007）
