作物学报2016，Vol.42Issue(11)：1647-1655,9.DOI:10.3724/SP.J.1006.2016.01647
RNAi介导SMV-P3基因沉默增强大豆对花叶病毒病的抗性
RNAi-mediatedSMV-P3 Silencing Increases Soybean Resistance toSoybean Mosaic Virus
摘要
Abstract
Soybean mosaic virus(SMV) is one of the most important diseases in major soybean production areas and has severe effects on soybean production and seed quality in China. Breeding disease-resistant varieties is the most economical and effective strategy to prevent and control SMV. In this study, RNAi fragments of the gene encoding P3 protein, which is involved in SMV mobility and affecting host range, were introduced into soybean by plant-mediated RNA interference (RNAi) techniques to ex-plore the influence of RNAi-mediatedSMV-P3 silencing on soybean SMV resistance. Southern blot analysis revealed that exoge-nous RNAi fragments were integrated into the soybean genome at low copy numbers (1–4). T1–T3 generation transgenic soybeans were sprayed with herbicide and inserted fragments were examined using PCR. The results indicated that T-DNA insertion frag-ments could be stably inherited between generations of transgenic soybean. Inoculation of T2 and T3 generation transgenic soy-beans with SMV suggested that transgenic soybeans exhibited significantly higher resistance to the prevailing SMV strain, SC-3, in major soybean production areas than the non-transgenic control varieties Williams 82 and SN9. The disease index was reduced by 4.37–18.51. Further, the resistance could be stably inherited. In conclusion, RNAi-mediatedSMV-P3 silencing can significantly increase the SMV resistance of the transgenic soybean.关键词
大豆/大豆花叶病毒病/SMV-P3/RNAi介导基因沉默Key words
Soybean/Soybean mosaic virus/SMV-P3/RNAi-mediated gene silencing引用本文复制引用
杨向东,牛陆,张伟,杨静,杜茜,邢国杰,郭东全,李启云,董英山..RNAi介导SMV-P3基因沉默增强大豆对花叶病毒病的抗性[J].作物学报,2016,42(11):1647-1655,9.基金项目
本研究由国家转基因生物新品种培育科技重大专项(2016ZX08004-004)和吉林省科技发展计划项目(20150204011NY)资助。 This study was supported by the China National Major Project for Developing New GM Crops (2016ZX08004-004) and the Science & Technology Development Project of Jilin Province (20150204011NY)。 （2016ZX08004-004）
