作物学报2016，Vol.42Issue(11)：1721-1726,6.DOI:10.3724/SP.J.1006.2016.01721
小麦DELLA获得性突变体矮变1号增强了幼苗的抗盐能力
A Wheat DELLA Gain-of-function Mutant Aibian 1 Promotes Seedling Salt Tolerance
摘要
Abstract
DELLA proteins are negative regulators in GA signal pathway. DELLA protein is able to response to various environ-mental signals and plays an important role in plant resistance to biotic and abiotic stresses. The DELLA gain-of-function mutant Aibian 1 has been widely applied in wheat breeding for dwarfness, but its tolerance to salt is unclear. In this study, Aibian 1 was compared with Chinese Spring, Jing 411, and Aiganzao in salt tolerance using Hoagland’s hydroponic culture with 200 mmol L-1 NaCl. Total chlorophyll content, relative water content, superoxide dismutase (SOD) activity and malondialdehyde (MDA) con-tent were measured to evaluate the ability of salt tolerance. Immunoblot assay was employed to assess the DELLA protein abun-dance in wheat seedlings. After seven-day NaCl treatment, Aibian 1 showed no clear withering leaves, whereas the other three cultivars displayed obviously wilting and yellowing leaves. Among the four cultivars, Aibian 1 had the least loss on chlorophyll content and relative water content, the highest SOD activity, and the lowest levels of MDA content after salt treatment. Im-munoblot assay indicated the highest accumulation of DELLA protein in Aibian 1 among the four cultivars. Thus, we consider the high level of DELLA protein is closely related to salt tolerance in Aibian 1.关键词
小麦DELLA蛋白/NaCl胁迫/叶绿素含量/超氧化物歧化酶/丙二醛Key words
Wheat DELLA proteins/Salt tolerance/Chlorophyll content/Superoxide dismutase/Malondialdehyde引用本文复制引用
王润青,樊晓聪,宋梅芳,肖阳,郭林,孟凡华,杨青华,吴大付,杨建平..小麦DELLA获得性突变体矮变1号增强了幼苗的抗盐能力[J].作物学报,2016,42(11):1721-1726,6.基金项目
国家重点研发计划项目(七大作物育种试点专项)(2016YFD101802),北京市自然科学基金重点项目(6151002)和中国农业科学院科技创新工程项目的资助。 This study was supported by the National Key R&D Program of China (Pilot Project on Seven Main Crop Breeding)(2016YFD101802), the Natural Science Foundation of Beijing (Key Program)(6151002) and the Agricultural Science and Technology Innovation Program of CAAS （七大作物育种试点专项）
