安徽农业科学2016，Vol.44Issue(28)：3-6,4.
产淀粉酶菌种的分离纯化及培养条件研究
S tudy on Separ ation, Purification and Culture Conditions of an Amylase Producing Strain
纪韦韦 1张小华 1高静1
作者信息
- 1. 江苏农林职业技术学院，江苏句容212400
- 折叠
摘要
Abstract
Objective] To explore the separation, purification and culture conditions of an amylase producing strain.[Method] Bacterium A, which had a strong ability to produce amylase, was isolated from the soil and then purified and cultured.[ Result] Bacterium A had the stron-gest ability to produce amylase in the medium using starch as the carbon source, and the ratio of the average diameter of hydrolyzation circles R2 to the average diameter of the strain R1(called R2/R1 for short) was 2.69;when being cultured in the medium using sodium nitrate as the nitrogen source, bacterium A had the strongest ability to produce amylase, and R2/R1 was 2.69;as the pH of the medium was 7.5, the ability to produce amylase was the strongest, and R2/R1 was 2.95.[Conclusion] Bacterium A should be cultured in the medium where starch and so-dium nitrate are as the carbon source and the nitrogen source and pH is 7.5 to improve the ability to produce amylase.关键词
淀粉酶/培养基/培养条件Key words
Amylase/Medium/Culture conditions分类
生物科学引用本文复制引用
纪韦韦,张小华,高静..产淀粉酶菌种的分离纯化及培养条件研究[J].安徽农业科学,2016,44(28):3-6,4.
