安徽农业科学2016，Vol.44Issue(28)：17-18,29,3.
福林试剂法快速检测藤仓赤霉发酵液中赤霉酸含量
Quick Detection of Gibberellins in Gibberella fujikuroiF ermentation Broth Using Folin-Ciocalteu’s Phenol Reagent
周圣骄 1宋志远 2聂志奎1
作者信息
- 1. 江西新瑞丰生化有限公司，江西新干331300
- 2. 江西省植物生长调节剂工程研究中心，江西新干331300
- 折叠
摘要
Abstract
Objective] The aim was to optimize reaction conditions of Folin-Ciocalteu’s phenol reagent.[Method] With high-yield Gibberella fujikuroi F12 as the research strain which was isolated from large quantities of UV mutation samples, Folin-Ciocalteu’ s phenol reagent method was used to quickly detect the gibberellin acid ( GA3 ) yield in Gibberella fujikuroi fermentation broth by the change of absorbance.[ Result] When 1.5 mL Folin-Ciocalteu’s phenol reagent, 3.0 mL hydrochloric acid as well as 20 min heating time was selected, the final solution absor-bance had the best stability and still remained basically unchanged within 4 h.[Conclusion] The optimized method can quickly and accurately detect the GA3 yield in Gibberella fujikuroi fermentation broth.关键词
赤霉酸/福林试剂/快速检测法/藤仓赤霉菌/吸光值Key words
GA3/Folin’s reagent/Quick detection/Gibberella fujikuroi/Absorbance分类
农业科技引用本文复制引用
周圣骄,宋志远,聂志奎..福林试剂法快速检测藤仓赤霉发酵液中赤霉酸含量[J].安徽农业科学,2016,44(28):17-18,29,3.
