安徽农业科学2016，Vol.44Issue(28)：94-95,98,3.
气相色谱法测定蝉花中角鲨烯的含量
Determination of Squalene in Cordyceps cicadae Shing by Gas Chromatography
摘要
Abstract
Objective] To establish a method of capillary gas chromatography to detect the content of squalene in Cordyceps cicadae.[Method] Squalene in Cordyceps cicadae Shing was detected by gas chromatography.And the squalene contents in different parts of C.cicadae were com-pared.The optimal condition for the detection of squalene in C.cicadae was obtained.[Result] C.cicadae (1 g) was extracted by ultrasonic ex-traction,the product was methyl esterified at 40 ℃for 5 min by 1 mL of 0.5 mol/L sodium hydroxide-methanol solution and then extracted by n-hexane.The squalene could be determined quantitatively by GC.When the squalene concentration was from 21.43 to 214.30 μg/mL,there was a good linear relation (r=0.999 2) and a perfect method precision (RSD =3.71%),and the recovery rate was 77.80%-97.32%,and the aver-age recovery rate was 88.10% (n=9).[Conclusion] This method was simple and rapid with low pollution and high sensitivity,and it is suitable for determination of squalene content in C.cicadae.关键词
蝉花/角鲨烯/气相色谱法Key words
Cordyceps cicadae Shing/Squalene/Gas chromatography分类
轻工纺织引用本文复制引用
凡军民,贾君,谢春芹,韩艳丽..气相色谱法测定蝉花中角鲨烯的含量[J].安徽农业科学,2016,44(28):94-95,98,3.基金项目
江苏农林职业技术学院科研基金项目（2015 kj034）；创新团队科研基金项目（2014td002）。 （）
