大豆科学2016，Vol.35Issue(5)：800-804,5.DOI:10.11861/j.issn.1000-9841.2016.05.0800
大豆异黄酮合成关键酶基因对蚜虫取食的防御响应分析
The Analysis of Key Gene of Soybean Isoflavone Synthesis Response to Aphid Feeding Defense
摘要
Abstract
In order to elucidate isoflavone mechanisms of the response to aphid in the soybean plants,different-resistant soybean varieties PI567598B (high),Suinong 30 (medium),Dongnong 51 (low) and Williams 82 (flu),were inoculated by soybean aphid for 0,7,14 and 21 days.Using qPCR,the expression patterns of key enzyme genes were analyzed in response to aphid feeding defense,including Phenylalanine ammonia lyase (PAL),Flavanone hydroxylase-3-3 (F3H),Isoflavone synthase gene 1 (ISF1) and Isoflavone synthase gene 2 (ISF2).The results showed leaves from PI567598B had higher PAL,F3H,ISF1 and ISF2 expression than Williams 82 without aphid attack.The PAL gene in PI567598B and Suinong 30 was increased significantly after inoculated 14 d and reached highest level at 21 d.The F3H gene was increased rapidly at 7 d and then showed a decreasing trend;the IFS2 gene was increased rapidly and the highest in 24 h.The PAL,IFS2 and F3H genes in Dongnong 51 were not induced significantly after inoculated 7 d.The PAL and IFS2 genes in Williams 82 were not induced significantly and lower than other three lines even after being damaged by aphid,while gene expression of F3H decreased after inoculation.The expression of IFS1 gene was not induced in four lines after inoculation.These result showed that the expression level of isoflavone key enzyme gene were higher and longer in resistant varieties than that in susceptible soybean varieties,which was consistent with its resistance differences.关键词
大豆/异黄酮/关键基因/大豆蚜虫/防御响应Key words
Soybean/Isoflavones/Key Genes/Soybean Aphid/Defense Response分类
农业科技引用本文复制引用
李娜,于希森,李琪,李涵哲,李洋,徐婷婷,孟凡立..大豆异黄酮合成关键酶基因对蚜虫取食的防御响应分析[J].大豆科学,2016,35(5):800-804,5.基金项目
国家自然科学基金青年基金(31201229) （31201229）
转基因大豆新品种培育重大专项(2011ZX08004-004) （2011ZX08004-004）
中国博士后启动基金(2012M510914) （2012M510914）
黑龙江省博士后资助项目(LBH-Z11223) （LBH-Z11223）
东北农业大学博士启动基金(2012RCB07). （2012RCB07）
