动物营养学报2016，Vol.28Issue(10)：3101-3109,9.DOI:10.3969/j.issn.1006-267x.2016.10.012
饲粮精粗比对泌乳水牛瘤胃细菌和甲烷菌区系的影响
Dietary Forage to Concentrate Ratio Affects Ruminal Bacterial and Methanogen Community Composition of Water Buffaloes
摘要
Abstract
This study was conducted to study the effects of dietary forage to concentrate ratio on ruminal bacte-rial and methanogen community composition of water buffaloes. Fifteen healthy similar hybrid water buffaloes were selected and divided into three groups with 5 heads per group according to body weight and feed intake. The three groups were fed diets with concentrate to forage ratio at 0∶100 ( all forage group) , 35∶65 ( low con-centrate group) and 50∶50 ( medium concentrate group) , respectively. The total experimental period was 40 days, including 10 days of advanced experiment feeding period. On day 1 after the experiment, rumen fluid sample was taken from rumen through mouth, and ruminal fluid DNA was extracted to research ruminal bacte-rial and methanogen community by Illumina Miseq 250PE. The results showed as follows: 1) at phyla level, ruminal bacterial community of buffaloes was dominated by Bacteroidetes ( 45% to 65%) and Firmicutes ( 13% to 27%) and Proteinobacter ( 13% to 18%); compared with low concentrate group and medium con-centrate group, all forage group increased the proportions of Bacteroidetes, Fibrobacter and SR1, but decreased the proportions of Firmicutes, Verrucomicrobia, Actinobacteria and Chloroflexi;at the family level, the domi-nant bacteria were Prevotellaceae ( 15% to 32%) and Fibrobacteraceae ( 8% to 21%);compared with medium concentrate group, all forage group and low concentrate group increased the proportions of Prevotellaceae, while decreased the proportions of Fibrobacteraceae, Lachnospiraceae, Ruminococcaceae, Coriobacteriaceae and Bifidobacteriaceae; additionally, the bacterial community in medium concentrate group was more diverse than that in all forage group. 2) Ruminal methanogen community of water buffaloes was dominated by Metha-nobrevibacter ( over 90%) , followed by Thermoplasma; dietary concentrate to forage ratio had no influence on ruminal methanogen community of water buffaloes. In conclusion, bacteria related with fiber digestion tends to be increased by all forge diet, but ruminal bacterial diversity is decreased by all forage diet;there is no influ-ence of concentrate to forage ratio on ruminal methanogen composition of water buffaloes at genus level.关键词
水牛/精粗比/瘤胃/细菌区系/甲烷菌区系Key words
water buffalo/concentrate to forage ratio/rumen/bacteria community/methanogen community分类
农业科技引用本文复制引用
林波,梁辛,李丽莉,韦升菊,李萍,邹彩霞..饲粮精粗比对泌乳水牛瘤胃细菌和甲烷菌区系的影响[J].动物营养学报,2016,28(10):3101-3109,9.基金项目
国家自然科学基金(31160470) （31160470）
广西自然科学基金(2013GXNSFBA019114) （2013GXNSFBA019114）
