动物营养学报2016，Vol.28Issue(10)：3110-3122,13.DOI:10.3969/j.issn.1006-267x.2016.10.013
不同脂肪和葡萄糖水平对大黄鱼生长性能、肝脏糖酵解和糖异生关键酶活性的影响
Effects of Different Lipid and Glucose Levels on Growth Performance, Hepatic Glycolysis and Gluconeogenic Key Enzyme Activities of Large Yellow Croaker (Larmichthys crocea Richardson)
摘要
Abstract
An 8-week feeding trial was conducted to evaluate the effects of different lipid and glucose levels on growth performance, hepatic glycolysis and gluconeogenic key enzyme activities, serum biochemical indices, glycogen contents and digestive enzyme activities etc. of large yellow croaker. Six experimental diets were for-mulated to contain two lipid levels ( 5% and 10%) and three glucose levels ( 10%, 20% and 30%) using 2 × 3 double-factor experimental design. Each diet was randomly assigned to 3 replicates of 50 juvenile large yellow croakers with the initial body weight of ( 14. 79 ± 0. 13 ) g. The results showed that weight gain rate ( WGR) , specific growth rate ( SGR) and feed efficiency ( FE) were not significantly affected by the interac-tion of dietary lipid and glucose levels (P>0.05). When the dietary lipid level was 5%, the WGR and SGR were decreased with the increase of dietary glucose level, which in 30% glucose group were significantly lower than those in 10% glucose group ( P<0.05) . The interaction of dietary lipid and glucose levels had significant effects on the contents of crude lipid and moisture of whole body ( P<0.05) , but had no significant effects on the content of crude protein of whole body ( P>0.05) . The contents of hepatic glycogen and muscle glycogen were significantly affected by the interaction of dietary lipid and glucose levels (P<0.05). When the dietary lipid level was 5%, hepatic glycogen content was increased with the dietary glucose level increasing, while the muscle glycogen content was firstly increased and then down. But when the dietary lipid level was 10%, hepat-ic glycogen content was firstly increased and then decreased with the dietary glucose level increasing, while the muscle glycogen content was increased. The interaction of dietary lipid and glucose levels did not have signifi-cant effects on the contents of total protein, total cholesterol, triglyceride, glucose and the activities of alanine aminotransferase and aspartate transaminase in serum of large yellow croaker (P>0.05). However, when the dietary lipid level was the same, serum glucose content had an increase trend with dietary glucose level increas-ing, and that in 30% glucose group was significantly higher than that in 10% glucose group ( P<0.05) . Hepat-ic glucokinase, phosphofructokinase and phosphoenolpyruvate carboxy kinase activities were significantly af-fected by the interaction of dietary lipid and glucose levels ( P<0.05) , while there were no significant effects on pyruvate kinase, glucose-6-phosphatase and fructose-1,6-bisphosphatase activities ( P>0.05) . When the di-etary lipid level was 10%, the hepatic glucokinase and phosphofructokinase activities were increased with dieta-ry glucose level increasing, while the hepatic phosphoenolpyruvate carboxy activity was firstly increased and then decreased. These results demonstrate that, compared with the dietary 10% lipid level, when the dietary lipid level is 5%, large yellow croaker has the ability to maintain the balance of blood glucose content by adjus-ting hepatic glycogen contents, glucose metabolism key enzyme activities and effectively utilizes glucose in di-ets with the dietary glucose level increasing. The results of present study indicate that the optimal requirements of lipid and carbohydrate for large yellow croaker in the larval stage are recommended to be 10% and 20%, re-spectively.关键词
大黄鱼/葡萄糖/脂肪/生长性能/糖酵解关键酶/糖异生关键酶Key words
large yellow croaker ( Larmichthys crocea Richardson )/glucose/lipid/growth performance/glycolysis key enzymes/gluconeogenic key enzymes分类
农业科技引用本文复制引用
马红娜,周飘苹,陆游,袁野,侯迎梅,孙蓬,丁立云,周歧存..不同脂肪和葡萄糖水平对大黄鱼生长性能、肝脏糖酵解和糖异生关键酶活性的影响[J].动物营养学报,2016,28(10):3110-3122,13.基金项目
国家自然科学基金项目(31272670) （31272670）
宁波市农业科技攻关重大项目(2012C10025) （2012C10025）
国家科技部星火重大计划项目(2014GA701001) （2014GA701001）
浙江省重中之重一级学科(水产)开放基金项目 （水产）
