动物营养学报2016，Vol.28Issue(10)：3123-3133,11.DOI:10.3969/j.issn.1006-267x.2016.10.014
饲料亚麻酸含量对大规格鲈鱼生长性能、抗氧化指标和血清生化指标的影响
Effects of Dietary α-Linolenic Acid Content on Growth Performance, Antioxidant Indices and Serum Biochemical Indices of Large Size Japanese Seabass (Lateolabrax japonicus)
摘要
Abstract
A 12-week feeding experiment was conducted to evaluate the effects of dietaryα-linolenic acid ( ALA) content on growth performance, antioxidant indices and serum biochemical indices of large size Japanese seabass ( Lateolabrax japonicus) , in order to get the optimal dietary ALA content for large size Japanese seabass. Six iso-nitrogenous and isoenergetic diets were formulated with dietary ALA content of 0. 06%, 0.99%, 2.03%, 3. 18%, 4.12% and 5.08% dry weight by adding perilla oil in a basal diet, respectively. Japanese seabass with the initial body weight of (207.77±0.64) g were fed experimental diets, and each diet had 3 replicates with 20 fish in each replicate. The results showed as follows:1) the specific growth rate ( SGR) and feed efficiency ( FE) increased at first and then decreased with the increase of dietary ALA content, and reached their peaks at the 2. 03% ALA group, but no significant differences were found in SGR and FE between 2.03%and 3.18%ALA groups ( P>0.05) . The highest values of hepatosomatic index ( HSI ) and viscerasomatic index ( VSI ) were found in 5. 08% ALA group, and significantly higher than those in 0.06% ALA group ( P<0.05) . 2) The body ether extract content in-creased with increase of dietary ALA content, while the body crude protein content increased at first and then de-creased with the increase of dietary ALA content. The body crude protein content in 4.12%and 5.08%ALA groups was significantly lower than that in 0.06% ALA group (P<0.05), while the body ether extract content in 4.12%and 5.08% ALA groups was significantly higher than that in 0.06%, 0.99%, 2.03% and 3.18% ALA groups ( P<0.05) . No significant differences were found in body moisture and ash contents among groups ( P>0.05) . 3) The activity of superoxide dismutase ( SOD) in serum and liver in 2.03%ALA group had no significant difference com-pared with 3.18% ALA group ( P>0.05) , but significantly higher than that in 0.06% and 5.08% ALA groups ( P<0.05). The malondialdehyde (MDA) content in serum in 2.03% and 3.18% ALA groups was significantly lower than that in 0.06%and 5.08%ALA groups ( P<0.05) , and the MDA content in liver in 2.03%ALA group was the lowest, which significantly lower than that in other groups except 3.18% ALA group ( P<0.05) . 4) The activities of glutamic-oxaloacetic transaminase ( GOT) and glutamic-pyruvic transaminase ( GPT) in serum in 2.03% ALA group was the lowest, which significantly lower than those in 0.06% and 5.08% ALA groups ( P<0.05) . The con-tents of triglycerides ( TG) and cholesterol ( CHOL) in serum reached their peaks in 4.12%ALA group ( P<0.05) , and significantly higher than those in 0.06%ALA group ( P<0.05) . The high density lipoprotein cholesterol ( HDL-C) content in serum firstly increased and then showed a declining tendency with the increase of dietary ALA con-tent, and that in 2.03%, 3.18% and 4.12% ALA groups was significantly higher than that in other groups (P<0.05) . These results indicate that optimal dietary ALA content can improve the growth of large size Japanese sea-bass, and increase the antioxidant ability and liver health level. The broken-line model analysis based on SGR and FE as evaluation indices indicate that the optimal dietary ALA content is 2.53% and 2.72% dry weight of diet for Japanese seabass (body weight:207.77 to 406.94 g), respectively.关键词
大规格鲈鱼/亚麻酸/生长性能/血清生化指标/抗氧化能力Key words
large size Japanese seabass ( Lateolabrax japonicus)/α-linolenic acid/growth performance/serum biochemical indices/antioxidant ability分类
农业科技引用本文复制引用
王成强,徐后国,梁萌青,郑珂珂,柳茜..饲料亚麻酸含量对大规格鲈鱼生长性能、抗氧化指标和血清生化指标的影响[J].动物营养学报,2016,28(10):3123-3133,11.基金项目
农业公益性行业专项(201003200) （201003200）
中国博士后科学基金第八批特别资助项目(2015T80763) （2015T80763）
