动物营养学报2016，Vol.28Issue(10)：3134-3144,11.DOI:10.3969/j.issn.1006-267x.2016.10.015
圆斑星鲽幼鱼饲料中适宜的维生素C含量
Optimal Dietary Vitamin C Content for Juvenile Spotted Halibut (Verasper variegates)
摘要
Abstract
This experiment was conducted to study the effects of dietary vitamin C content on growth and the functions of digestion, absorption, antioxidant and immune of juvenile spotted halibut ( Verasper variegates) , in order to get the optimal dietary vitamin C content for juvenile spotted halibut. Water temperature was main-tained at (12.5±1.5) ℃ in this experiment. Juvenile spotted halibut with the body weight of (38.0±0.8) g were randomly assigned into 7 groups with 3 replicates per group and 30 fish per replicate, and fed 7 experi-mental diets with the vitamin C content were 10.2, 249.1, 402.8, 616.2, 769.5, 909.4 and 1 177.8 mg/kg, respectively. The experiment lasted for 8 weeks. The results showed as follows: the highest weight gain rate, feed efficiency ratio and feeding rate were found in 616. 2 mg/kg vitamin C group. The weight gain rate of 616.2 mg/kg vitamin C group was significantly higher than that of 10.2 and 909.4 mg/kg vitamin C groups ( P<0.05) , the feed efficiency ratio of 616.2 mg/kg vitamin C group was significantly higher than that of oth-er groups ( P<0.05) , and the feeding rate of 616.2 mg/kg vitamin C group was significantly higher than that of other groups except 1 177.8 mg/kg vitamin C group ( P<0.05) . The fish had the maximal liver and muscle vitamin C accumulation when dietary vitamin C content was 769.5 mg/kg. Serum malondialdehyde content of 616.2 and 769.5 mg/kg vitamin C groups was significantly lower than that of other groups except 402.8 mg/kg vitamin C group (P<0.05). The activities of serum catalase and lysozyme in 402.8 mg/kg vitamin C group had the highest values, which were significantly higher than those of other groups ( P<0.05) . Serum superox-ide dismutase activity of 402.8, 616.2 and 769.4 mg/kg vitamin C groups was significantly higher than that of other groups ( P<0.05) . Protease, amylase and lipase activities in stomach and intestine were at a high level when dietary vitamin C content was 402.8, 616.2 or 769.4 mg/kg. Dietary vitamin C content within the scope of 249.1 to 616.2 mg/kg could promote the growth of the intestinal mucosa and increase the fold height and in-testinal wall thickness. These results suggest that dietary vitamin C content within the scope of 402. 8 to 616.2 mg/kg can effectively meet the need of growth, and improve the antioxidant capacity, non-specific im-munity, digestion and absorption ability of juvenile spotted halibut.关键词
圆斑星鲽/维生素C/生长/免疫/抗氧化Key words
juvenile spotted halibut ( Verasper variegates)/vitamin C/growth/immunity/antioxidant分类
农业科技引用本文复制引用
王贞杰,陈四清,常青,刘长琳,胡建成,严俊丽,卢斌..圆斑星鲽幼鱼饲料中适宜的维生素C含量[J].动物营养学报,2016,28(10):3134-3144,11.基金项目
山东省自主创新成果转化专项(2013ZHZX2A0803) （2013ZHZX2A0803）
鳌山科技创新计划(2015ASKJ02-03) （2015ASKJ02-03）
