动物营养学报2016，Vol.28Issue(10)：3264-3274,11.DOI:10.3969/j.issn.1006-267x.2016.10.030
敌草快和硫辛酸对育肥猪肠道结构及消化功能的影响
Effects of Diquat and Lipoic Acid on Intestinal Morphology and Digestive Function of Finishing Pigs
摘要
Abstract
This study was to investigate the effects of diquat and lipoic acid ( LA ) on intestinal morphology and digestive function of finishing pigs, in order to explore whether diquat induced stress on the LA had a role in alleviating. Twenty-four healthy finishing pigs (Yorkshire) with the body weight of (70.64±3.61) kg were randomly allocated to control, LA, diquat, LA+diquat groups with 6 replicates per group and 1 pig per repli-cate by a 2×2 factorial arrangement. The trial period was 29 days. LA was given all in the trial (800 mg/kg di-et) . Diquat challenged pigs were given a single intraperitoneal injection of diquat at the 15th days and non-chal-lenged pigs were injected intraperitoneally with the equal physiological saline amount at the same time. All the pigs were slaughtered after the vena cava blood was sampledon the 29th day. Using kits to detected the contents of plasma and intestine oxidative damage markers and the activities of digestive enzymes in jejunum chyme. Af-ter hematoxylin and eosin ( HE ) staining, using image analysis software to observed intestinal morphology structure and determined villus height, crypt depth, and calculated the value of the villus height to crypt depth ( V/C) . Using fluorescence quantitative PCR to detected the relative expression abundance of occludin and zonula occludens protein-1 ( ZO-1) mRNA in jejunum. The results showed as follows:1) diquat significantly increased the contents of malondialdehyde ( MDA) , protein carbonyl ( PCO) and 8-hydroxy deoxyguanosine ( 8-OHdG) in plasma and intestine ( P<0.01) , significantly reduced the villus height and crypt depth in duode-num and jejunum and ileum ( P<0.01) , while significantly increased the V/C in duodenum and jejunum ( P<0.01) , and significantly increased the V/C of the ileum ( P<0.05) , significantly reduced the relative expres-sion abundance of occludin and ZO-1 mRNA in jejunum ( P<0.01) , and significantly reduced the activities of amylase, trypsase and lipase in jejunum chyme ( P<0.01) . 2) There were no significant difference on the con-tents of MDA, PCO and 8-OHdG in plasma and intestine when dietary supplementation of LA ( P>0.05) , as same as the villus height, crypt depth and V/C in duodenum, jejunum and ileum, the relative expression abun-dance of occludin and ZO-1 mRNA in jejunum, the activities of amylase, trypsase and lipase in jejunum chyme ( P>0.05) . 3) Under the oxidative stress, dietary supplementation of LA significantly reduced the content of 8-OHdG in plasma ( P<0.05) , while significantly improved the villus height in duodenum and jejunum ( P<0.05) , and significantly improved crypt depth in ileum ( P<0.05) , significantly reduced V/C in jejunum ( P<0.05) and ileum (P<0.01), significantly increased the relative expression abundance of occludin and ZO-1 mRNA in jejunum (P<0.05), significantly improved the activity of amylase and trypsase in jejunum chyme ( P<0.05) . In conclusion, diquat caused oxidative stress in pigs is strong, leading to serious injury of intestinal structure and weakened intestinal digestive function. Dietary supplementation of 800 mg/kg of LA can suppress the oxidative stress caused by diquat injection on intestinal injury of finishing pigs, and may improve digestive function.关键词
敌草快/硫辛酸/育肥猪/氧化应激/肠道Key words
diquat/lipoic acid/finishing pigs/oxidative stress/intestine分类
农业科技引用本文复制引用
鲍伟光,郝月,崔艳军,李洁蕾,张校军,李淦,王占彬,顾宪红..敌草快和硫辛酸对育肥猪肠道结构及消化功能的影响[J].动物营养学报,2016,28(10):3264-3274,11.基金项目
国家重点基础研究发展计划课题(2012CB124706) （2012CB124706）
国家科技支撑计划课题(2012BAD39B02) （2012BAD39B02）
中国农业科学院科技创新工程( ASTIP-IAS07) （ ASTIP-IAS07）
