动物营养学报2016，Vol.28Issue(10)：3301-3310,10.DOI:10.3969/j.issn.1006-267x.2016.10.034
谷氨酰胺及其二肽对过氧化氢诱导山羊瘤胃上皮细胞凋亡及凋亡相关基因表达的影响
Effects of Glutamine and Its Dipeptides on Apoptosis and Apoptosis Related Gene Expressions Induced by Hydrogen Peroxide in Ruminal Epithelial Cells of Goats
摘要
Abstract
This study was conducted to investigate the effects of glutamine ( Gln ) , glycyl-glutamine ( Gly-Gln) and alanyl-glutamine ( Ala-Gln) on apoptosis rate and gene expressions of Bcl-2 and Bax of apoptosis cells according to establish apoptosis models for ruminal epithelial cells of goats induced by hydrogen peroxide (H2O2). Subculture ruminal epithelium cells of 60 day-old Xiangdong black goats were selected and cultured with different concentrations [0(control group), 100, 400 and 800 μmol/L] of H2O2, and flow cytometry ( FCM) technique was used to detected cell apoptosis. Subculture ruminal epithelium cells were divided into 5 groups, control group and group 1 were cultured with 0 and 800 μmol/L H2O2, and groups 2, 3 and 4 were cultured with 800 μmol/L H2O2, meanwhile with 17.28 mmol/L Gly-Gln (group 2), 16.0 mmol/L Gln (group 3) and 16.0 mmol/L Ala-Gln (group 4). FCM technique was used to detected cell apoptosis, and gene expressions of Bcl-2 and Bax were detected by real-time fluorescent quantitative PCR. The results showed as follows:1) compared with control group, when the concentration of H2O2 reached 800 μmol/L, apoptosis rate of early apoptosis significantly increased ( P<0.05); apoptosis rate of late apoptosis firstly increased and then decreased with the increasing of H2O2 concentration, while compared with control group, experimental groups were all significantly increased ( P<0.05) . 2) Compared with control group, apoptosis rate of late ap-optosis in group 4 significant increased ( P<0.05) , and apoptosis rate of early apoptosis in experimental groups were all significantly higher than that in control group ( P<0.05) . 3) Compared with control group, Bcl-2/Bax in experimental groups was significantly increased (P<0.05); compared with group 1, Bcl-2/Bax in groups 2, 3 and 4 was significantly increased (P<0.05), and group 2 was significantly higher than groups 3 and 4 (P<0.05). In conclusion, Gly-Gln plays protection role in early apoptosis induced by H2O2 in ruminal epithe-lium cells of goats.关键词
湘东黑山羊/瘤胃上皮细胞/凋亡/凋亡基因Key words
Xiangdong black goat/ruminal epithelium cell/apoptosis/apoptosis gene分类
农业科技引用本文复制引用
韩奇鹏,掲红东,罗玲,王凯军,周传社,张佩华,孔志伟,汤少勋..谷氨酰胺及其二肽对过氧化氢诱导山羊瘤胃上皮细胞凋亡及凋亡相关基因表达的影响[J].动物营养学报,2016,28(10):3301-3310,10.基金项目
国家自然科学基金面上项目(31372342) （31372342）
国家科技支撑计划课题(2012BAD14B17) （2012BAD14B17）
中国科学院科技服务网络计划(STS计划)课题( KFJ-EW-STS-071) （STS计划）
