动物营养学报2016，Vol.28Issue(10)：3352-3358,7.DOI:10.3969/j.issn.1006-267x.2016.10.040
提取液及固-液分离方法对固态非淀粉多糖酶类活性的影响
Effects of Extraction Solution and Solid-Liquid Separation Method on the Activities of Non-Starch Polysaccharide Enzymes
摘要
Abstract
This experiment was conducted to investigate appropriate extract solution and solid-liquid separation method for evaluating enzyme in solid feed enzyme product. Extract solution of deionized water, acetic acid-so-dium acetate buffer solution (0.1 mol/L, pH 5.50), phosphate buffer solution (0.05 mol/L, pH 6.00) or 0.9% NaCl solution and solid-liquid separation method of no separation, 3 000 r/min of centrifugation for 3 min or filtration were used in a 4×3 factorial arrangement. Each treatment contained 5 replicates with 2 determi-nation in each. The activities of enzymes in the products were determined. Then, the effects of the types of ex-tract solution on the contents of solute and protein were investigated for the enzyme products ( excludingα-gal-actosidase) dissolved and centrifuged. The results showed that the highest determined activity of xylanase was presented in the phosphate buffer solution buffer solution ( P<0.05) . The similar determined activities ofβ-glu-canase were observed in acetic acid-sodium acetate buffer solution, phosphate solution and 0.9% NaCl solution ( P>0.05) , and were significantly higher than that in deionized water ( P<0.05) . The greatest and greater de-termined activities ofβ-mannanase were observed in deionized water and acetic acid-sodium acetate buffer solu-tion, respectively, and were significantly greater than those in phosphate solution or 0.9% NaCl solution ( P<0.05) . The type of extract solution had no significant effect on the determined activity of α-galactosidase ( P>0.05) . The solid-liquid separation method had no significant effect on the determined activity of xylanase ( P>0.05). The greater determined activity of β-glucanase was observed in centrifugation or filtration (P<0.05). The greatest determined activity of β-mannanase was presented in centrifugation (P<0.05). However, the highest determined activity of α-galactosidase product was observed in no separation (P<0.05). There was a significant interaction between the type of extract solution and solid-liquid separation method in the determined activities of 4 enzyme products ( P<0.01) . The highest dry matter solubility of xylanase product was observed in acetic acid-sodium acetate buffer solution ( P<0.05) . The highest dry matter solubility ofβ-glucanase andβ-mannanase was presented in deionized water and 0.9% NaCl solution ( P<0.05) . However, the lowest protein contents of xylanase,β-glucanase andβ-mannanase were observed in acetic acid-sodium acetate buffer solution ( P<0.05) . It is concluded that acetic acid-sodium acetate buffer solution is the most efficient to extract the en-zyme protein from the product. After dissolved, the α-galactosidase product is not suitable to separate, but the other enzymes can be separated with centrifugation.关键词
非淀粉多糖酶/提取液/固-液分离/酶活性Key words
non-starch polysaccharide enzymes/extract solution/solid-liquid separation/enzyme activity分类
农业科技引用本文复制引用
廖睿,赵峰,齐智利,张宏福..提取液及固-液分离方法对固态非淀粉多糖酶类活性的影响[J].动物营养学报,2016,28(10):3352-3358,7.基金项目
国家自然科学基金项目(31172215) （31172215）
中国农业科学院科技创新工程(ASTIP-IAS07) （ASTIP-IAS07）
