中国耳鼻咽喉头颈外科2016，Vol.23Issue(9)：501-506,6.DOI:10.16066/j.1672-7002.2016.09.003
基于miRNAs芯片的喉鳞状细胞癌致癌机制的初步分析
Array profiling identified MiRNAs dysregulation in laryngeal squamous cell carcinoma
摘要
Abstract
OBJECTIVE The purpose of this study was to analyze the screened miRNAs related to tumorigenesis using miRNA array in laryngeal squamous cell carcinoma (LSCC), and provide a set of miRNAs that may be useful for the development of novel diagnostic markers and/or more effective therapeutic strategies from the screened miRNAs in LSCC. METHODS A total number of 5 patients who underwent surgery for primary laryngeal squamous cell carcinoma were recruited for miRNA array analysis. LSCC tissues compared with corresponding adjacent non-neoplastic tissues were analyzed by the Affymetrix GeneChip miRNA Array 3.0 to screen effective miRNAs, and the raw dataset had been submitted to Gene Expression Omnibus. Then mirfocus 3.0 database was adopted to analyze putative regulated miRNAs related to MCM4, a gene related to tumorigenesis we had studied previously in LSCC. Moreover, the selected putative regulated miRNAs were also validated by qRT-PCR in another 21 patients diagnosed as LSCC. RESULTS Analyzed by the miRNAs arrays, there were 127 miRNAs significantly related to tumorigenesis, and 78 showed a higher expression in tumor than in non-tumor tissue while 49 presented the contrasting pattern (P<0.01). Then analyzed by mirfocus 3.0 database, there were 2 putative regulated miRNAs, hsa-miR-24-3p and hsa-miR-183-5p, related to the expression of MCM4. Another miRNA we should focus on was hsa-miR-30a-5p, which was down-expressed obviously analyzed by the miRNA array. The expression of the 3 putative regulated miRNAs were also validated by qRT-PCR in another 21 patients, and the result was the same with that in miRNA array (P<0.05). CONCLUSION The 3 putative miRNAs based on miRNA array analysis, hsa-miR-24-3p, hsa-miR-183-5p and hsa-miR-30a-5p, could be considered as potential diagnostic and therapeutic markers in LSCC. The result will contribute to the understanding of the molecular basis of LSCC and help to improve the treatment.关键词
肿瘤,鳞状细胞/微RNAs/芯片分析技术/计算生物学Key words
Neoplasms, Squamous Cell/MicroRNAs/Microchip Analytical Procedures/Computational Biology引用本文复制引用
廉猛,刘红刚,翟杰,王茹,王海舟,冯凌,时倩,马泓智,房居高,刘晓琴..基于miRNAs芯片的喉鳞状细胞癌致癌机制的初步分析[J].中国耳鼻咽喉头颈外科,2016,23(9):501-506,6.基金项目
北京市医管局扬帆计划（XMLX201311）、北京市科委重点项目（Z141107002514003）和内蒙古自治区自然科学基金面上项目（2015MS0834）联合资助 （）
