中国耳鼻咽喉头颈外科2016，Vol.23Issue(10)：585-589,5.DOI:10.16066/j.1672-7002.2016.10.008
顺铂引起斑马鱼侧线毛细胞凋亡的实验研究
Cell death type of zebrafish lateral line hair cellsinduced by cisplatin
芈肖肖 1孔肖雯 1李圆 2施军平1
作者信息
- 1. 杭州师范大学附属医院转化医学平台，浙江 杭州 310015
- 2. 杭州师范大学附属医院耳鼻咽喉头颈外科，浙江 杭州 310015
- 折叠
摘要
Abstract
OBJECTIVE To explore cell death type of lateral line hair cellsinduced by cisplatin in zebrafish. METHODS Zebrafish larva were incubated in 1mM cisplatin solution for 6 hrs to induce about 90%lateral line hair cells loss. Time lapse imaging was used to detect the morphology of cisplatin-incubated hair cells in wildtypezebrafish pre-labelled by live dyes Bodipy TR C5-ceramide and Sytogreen 24. TUNEL assay and In situ anti-active Caspase-3 antibody staining were performed to detect nuclei fragmentation and Caspase-3 activity respectively. RESULTS Compared to control group, hair cells condensationand nuclei fragmentation (P<0.05) were detected in cisplatin-incubated group, and active Caspase-3 activity was also observed after cisplatin addition. CONCLUSION Cisplatinmay induced zebrafish lateral line hair cells loss by Caspase-3-dependent apoptotic pathway.关键词
动物,实验/顺铂/毛细胞/细胞凋亡Key words
Animals, Laboratory/Cisplatin/Hair Cell/Apoptosis引用本文复制引用
芈肖肖,孔肖雯,李圆,施军平..顺铂引起斑马鱼侧线毛细胞凋亡的实验研究[J].中国耳鼻咽喉头颈外科,2016,23(10):585-589,5.
