南方农业学报2016，Vol.47Issue(9)：1506-1511,6.DOI:10.3969/jissn.2095-1191.2016.09.1506
广西甘蔗鞭黑粉菌遗传多样性分析
Genetic diversity of Sporisorium scitamineam in Guangxi
摘要
Abstract
[Objective]Genetic diversity of Sporisorium scitamineam from main sugarcane growing area of Guangxi was studied, in order to provide new ideas for comprehensive control of sugarcane smut. [Method]S. scitamineam was collected from main sugarcane growing areas of Guangxi, and genomic DNA were extracted from 70 haploid strains. Optimized random amplified polymorphic DNA(RAPD) reaction system and 10 RAPD primers screened deliberately were used to de-tect polymorphism of S. scitamineam by PCR amplification. Then, according to electrophoretogram, Cluster analysis was conducted to construct phylogenic tree with NTSYS2.1 software. [Result]The results showed that, 10 RAPD primer pair could amplify 4-11 bands each, with a total of 77 bands, each band ranged from 150 to 3000 bp in length, but only 6 polymorphic sites were detected, which accounted for a polymorphic ratio of 7.79% in population. The genetic similarity coefficient of 70 S. scitamineam strains ranged from 0.96 to 1.00, and those strains were clustered into 2 clusters at genet-ic similarity level of 0.96, the first cluster was comprised of 2 strains collected from Xiaoyi town of Hengxian county, while the remaining 68 stains were clustered into the second cluster. [Conclusion]S. scitamineam is at low level of genetic differentiation in Guangxi. Polymorphism of S. scitamineam had no significant correlation with its geographical origin, hosts and mating types.关键词
甘蔗鞭黑粉菌/RAPD/遗传多样性/广西Key words
Sporisorium scitamineam/RAPD/genetic diversity/Guangxi分类
农业科技引用本文复制引用
林珊宇,贤小勇,颜梅新,朱桂宁..广西甘蔗鞭黑粉菌遗传多样性分析[J].南方农业学报,2016,47(9):1506-1511,6.基金项目
广西主席科技基金项目（11166-02）；广西农业科学院科技发展基金项目（桂农科2013JQ20）；广西作物病虫害生物学重点实验室开放基金项目 （）
