南方农业学报2016，Vol.47Issue(9)：1558-1563,6.DOI:10.3969/jissn.2095-1191.2016.09.1558
响应面法优化葫芦多糖提取条件
Optimization for extraction process of polysaccharide from Lagenaria siceraria(Molina) Standl. by response surface methodology
摘要
Abstract
[Objective]The extraction process of polysaccharides from Lagenaria siceraria(Molina) Standl. were opti-mized by using response surface methodology, in order to provide technical support for further study of polysaccharides from L. siceraria. [Method]With L. siceraria as raw materials, based on single factor experiment, the extraction temper-ature, liquid-solid ratio and extraction time were selected as independent variables. Response surface methodology based on a central composite design with 3 factors and 4 levels was applied to analyze effects of 3 factors and their interactions on extraction rate of polysaccharides , so as to optimize extraction conditions. [Result]The results showed that, the quadratic multinomial regression equation was established as:Y=5.54+0.14A+0.43B+0.36C+0.016AB-0.081AC-0.13BC-0.34A2-0.23B2-0.21C2(R2=0.9956; A: extraction temperature; B: liquid-solid ratio; C: extraction time). The 3 factors af-fecting extraction rate was in order as follows: liquid-solid ratio>extraction time>extraction temperature. Three factors and interaction between liquid-solid ratio and extraction time had extremely significant effect on extraction rate of polysaccha-rides(P<0.01), and the interaction between extraction temperature and extraction time also had significant effect(P<0.05). The optimum extraction process was as follows: extraction temperature of 83 ℃, liquid-solid ratio ratios of 24∶1(mL/g), extraction time of 2.3 h, extracting polysaccharides twice. Under optimum condition, the extraction rate of polysaccharides was up to (5.810±0.240)%, which was close to the theoretical value(5.820%). [Conclusion]The response surface method-ology is workable to optimize extraction process of polysaccharides, the optimized process conditions can improve extrac-tion rate of polysaccharides from L. siceraria.关键词
葫芦/多糖/响应面法/提取条件优化Key words
Lagenaria siceraria(Molina) Standl./polysaccharide/response surface methodology/optimization of ex-traction condition分类
医药卫生引用本文复制引用
阿得力江·吾斯曼,买买提·艾孜子,阿依姑丽·买买提明,吐尔洪·努尔,赛福丁·阿不拉..响应面法优化葫芦多糖提取条件[J].南方农业学报,2016,47(9):1558-1563,6.基金项目
国家自然科学基金项目（31360625）；新疆产学研联合培养项目 （）
