华北农学报2016，Vol.31Issue(5)：35-43,9.DOI:10.7668/hbnxb.2016.05.006
棉花转录因子 GhSNAC3启动子的克隆与表达分析
The Cloning and Expression Analysis of Transcription Factor GhSNAC3 Promoter in Cotton
摘要
Abstract
In order to analyze the structure and function of the promoter of cotton NAC transcription factor gene GhSNAC3,the promoter fragment was cloned and identified from Lumianyan 32 in Gossypium hirsutum L..The cis-acting elements in the promoter sequence was predicted and analyzed by using the online database of Plant CARE, PLACER and BDGP.A plant expression vector consisting of GhSNAC3 and its promoter was constructed and trans-formed into Nicotiana tabacum L.to investigate the transcription activities of the promoter under different stress treatments.The results showed that besides the basic cis-acting elements such as CAAT-box,there existed several MeJA,GA and ABA-responsive elements as well as multiple light-responsive elements and stress-induced controlling elements in the promoter.Under salt stress GhSNAC3 had a high level of expression in the roots of transgenic tobac-co plants,while the expression level was low in the stems and leaves,suggesting that the gene GhSNAC3 might played an important role in stress responses in cotton,and its promoter might be tissue-specific and salt-inducible. The results provide the basis for the further study of cotton NAC signal pathway as well as salt tolerance improve-ment through genetic engineering in the future.关键词
棉花/NAC/启动子/盐诱导Key words
Cotton/NAC/Promoter/Salt-induced分类
生物科学引用本文复制引用
马骏骏,柳展基,李菲,王立国,傅明川,朱新霞,刘任重..棉花转录因子 GhSNAC3启动子的克隆与表达分析[J].华北农学报,2016,31(5):35-43,9.基金项目
国家自然基金项目（31571755）；山东省优秀中青年科学家科研奖励基金计划项目（BS2015 NY014）；山东省农业科学院青年基金项目 （）
