华北农学报2016，Vol.31Issue(5)：62-70,9.DOI:10.7668/hbnxb.2016.05.010
ssc-miR-486靶基因预测及生物信息学分析
Target Gene Prediction and Bioinformatics Analysis of ssc-miR-486
摘要
Abstract
The target gene prediction and bioinformatics analysis of ssc-miR-486 were performed to explore its effects on the porcine fat deposition.First,the Real-time PCR was applied to detect differential expression of ssc-miR-486 in the subcutaneous adipose tissue of Laiwu pig and Large White pig which were quite different in terms of fat deposition capability.Then we performed bioinformatics analysis of ssc-miR-486 including the prediction of the target genes and transcription factor binding sites,the analysis of sequence conservation and involved Gene Ontology and KEGG pathway of its target genes using the databases such as miRBase,Ensembl and NCBI and bioinformatic softwares such as miRanda,PITA,RNAhybrid,Cytoscape,JASPAR and Promoter Scan.ssc-miR-486 was significant-ly upregulated in the subcutaneous adipose tissue of Laiwu pig with easily fat accumulation.The bioinformatics anal-ysis showed that miR-486 was very conservative among various species and multiple transcription factor binding sites such as SREBP1 and SREBP2 were predicted in the promoter area of ssc-miR-486.Additionally the obtained 229 target genes of ssc-miR-486 were significantly enriched in many biological processes,for example lipid metabolism, lipid biosynthesis and cell metabolism,et al and signal transduction pathways such as steroid biosynthesis,inositol phospholipid metabolism and PPAR signaling pathway.The results suggest that ssc-miR-486 plays a certain role in fat deposition and lipid metabolism of pigs and provide a basis for fat deposition research in the future.关键词
ssc-miR-486/生物信息学/脂肪沉积/猪Key words
ssc-miR-486/Bioinformatics/Fat deposition/Pig分类
生物科学引用本文复制引用
张秀秀,黄万龙,郭云涛,苗向阳..ssc-miR-486靶基因预测及生物信息学分析[J].华北农学报,2016,31(5):62-70,9.基金项目
转基因生物新品种培育科技重大专项（2009ZX08008-004B；2008ZX08008-003）；国家“863”计划项目（2008AA10Z140）；国家自然科学基金项目（30571339）；中国农业科学院农业科技创新项目（ASTIP-IAS05）；国家重点基础研究发展计划（“973”计划项目）（2015 CB943100）；中国农业科学院创新基金项目（2004-院-1）；中央级公益性科研院所基本科研业务费专项资金项目 （）
