华北农学报2016，Vol.31Issue(5)：122-128,7.DOI:10.7668/hbnxb.2016.05.018
基于 SRAP 标记的花椒种质资源遗传多样性及群体结构分析
Genetic Diversity and Population Structure Analysis of Zanthoxylum Germplasms by SRAP
摘要
Abstract
To explore the genetic diversity and population structure of Zanthoxylum germplasm,the genetic di-versity among 269 accessions of Zanthoxylum which collected from Shaanxi,Shanxi,Yunnan,Sichuan and Gansu Provinces was studied by SRAP Markers.The results showed that 1 6 pairs of SRAP primes,amplified clear repetitive and intense bands,were selected from 1 20 combinations.A total of 1 69 clear bands were amplified,there were 1 51 polymorphic bands and the percentage of polymorphic bands was 93.8%;The UPGMA clustering showed that the populations were divided into two main groups,Z.armatum DC.and Z.bungeanum Maxim.The highest genetic di-versity level was observed within Shanxi Province,the minimum genetic distance was between Shaanxi and Gansu, the maximum was between Shaanxi and Yunnan.The AMOVA showed that the genetic variation was mainly caused by variation of intra-provenances (74%),rather than among provinces (26%);the results of population genetic structure were consistent with the UPGMA clustering and indicated obvious gene flow among provinces.The results provide a basis for the collection,classification and identification of Zanthoxylum resources.关键词
花椒/SRAP 分子标记/遗传多样性/遗传结构Key words
Zanthoxylum germplasms/SRAP Markers/Genetic diversity/Genetic structure分类
农业科技引用本文复制引用
李立新,杨途熙,魏安智,冯世静,陈旅,侯娜..基于 SRAP 标记的花椒种质资源遗传多样性及群体结构分析[J].华北农学报,2016,31(5):122-128,7.基金项目
国家林业局林业公益性行业科研专项（201304706）；西北农林科技大学产业技术集成与示范推广（TGZX2016-08）；贵州省林业厅中药现代化项目 （）
