环境工程学报2016，Vol.10Issue(10)：5593-5599,7.DOI:10.12030/j.cjee.201505199
N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化
Isolation and degradation characteristics of a N-vinylpyrrolidone-degrading strain ZF1 and its immobilization on bamboo-based carbon
摘要
Abstract
N-vinylpyrrolidone (NVP) is a common raw material widely used in many industries and belongs to the nitrogen heterocyclic compounds (NHCs).Biological degradation was demonstrated as the most cost effective and environmentally friendly way to remove nitrogen heterocyclic pollutants from the environment.In the current study,a highly effective NVP-degrading bacteria,designated ZFl,was isolated from activated sludge.Based on its morphological,physiological,and biochemical properties and 16S rRNA gene sequence,the strain was identified as Arthrobacter ureafaciens.Strain ZF1 could use NVP as its sole carbon and nitrogen sources,degrading 200 mg · L-1 NVP to a nondetectable level within 60 h.The optimal environmental conditions for the degradation of NVP were 30 ℃,initial pH of 7.0,and NaCl of 7 g · L-1.For its application in real wastewater treatment,strain ZF1 was immobilized onto bamboo-based carbon to enhance the NVP degradation efficiency.SEM observation indicated that strain ZF1 cells were attached well on the surface and pores of the bamboo-based carbon.The degradation kinetics of NVP by free and immobilized cells followed the first-order model.After it was reused 5 times,NVP degradation efficiency was maintained at 98％.关键词
N-乙烯基吡咯烷酮/分离鉴定/Arthrobacter ureafaciens/降解特性/竹炭/固定化Key words
N-vinylpyrrolidone/isolation and identification/Arthrobacter ureafaciens/degradation characteristics/bamboo-based carbon/immobilization分类
资源环境引用本文复制引用
郑菲菲,胡江,辛华东,陈立伟,蔡天明..N-乙烯基吡咯烷酮降解菌的筛选、降解特性及其竹炭固定化[J].环境工程学报,2016,10(10):5593-5599,7.基金项目
江苏省太湖水环境综合治理科研课题(TH2014210,TH2013210) （TH2014210,TH2013210）
