环境工程学报2016，Vol.10Issue(10)：5919-5923,5.DOI:10.12030/j.cjee.201603072
采用等电点沉淀法回收市政污泥水解液中的蛋白质
Protein recovery from hydrolysis solution of municipal sludge by isoelectric point precipitation
摘要
Abstract
Protein recovery from the waste sludge of municipal wastewater treatment plants is one of the new approaches to sludge resource recovery.The isoelectric point precipitation method is an efficient way to recover proteins from municipal sludge.The results of this study showed that the protein recovery rate was as high as 87.46％ at a pH of 3.5,precipitation temperature of 5 ℃,centrifugal speed of 3000 r · min-1,and protein concentration of 6000 mg · L-1.The amino acid content of the obtained sludge protein was analyzed by high performance liquid chromatography.It was found that seven essential amino acids (lysine,phenylalanine,methionine,threonine,isoleucine,leucine,and valine) accounted for 12.37％ of the protein recovery rate.The heavy metal content of the obtained sludge protein was analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES),demonstrating that the removal of As,Pb,and Cr was more than 90％ compared with that of the original municipal sludge.The isoelectric point precipitation method proved to be beneficial for the removal of heavy metals in municipal sludge,reducing the risk of heavy metal pollution in the protein product.关键词
市政污泥/蛋白质/等电点沉淀法/重金属Key words
municipal sludge/protein/isoelectric point precipitation method/heavy metal分类
资源环境引用本文复制引用
施正华,李秀芬,宋小莉,王新华,任月萍..采用等电点沉淀法回收市政污泥水解液中的蛋白质[J].环境工程学报,2016,10(10):5919-5923,5.基金项目
国家重点研发计划课题(2016YFC0400707) （2016YFC0400707）
国家水体污染控制与治理科技重大专项子课题(2015ZX07306001-5) （2015ZX07306001-5）
