环境工程学报2016，Vol.10Issue(10)：6042-6048,7.DOI:10.12030/j.cjee.201504252
一株假单胞菌(Pseudomonas)SYBS01降解石油的特性
Oil degradation characteristics of Pseudomonas SYBS01
摘要
Abstract
A petroleum degrading bacterial strain SYBS01 was isolated from oil-contaminated soil,and was identified as Pseudomonas sp.Oil degradation conditions were compared with single-factor and orthogonal experimental design,with the results showing that the optimum conditions were 35 ℃,170 r · min-1,yeast extract 15 g · L-1,KH2PO4 0.5 g · L-1,and a natural pH value.Among these,the yeast extract had the greatest influence on the oil-degradation rate.The oil-degradation rate of SYBS01 increased to 85.6％ four days after yeast extract had been added to the system,and was 6.7,7.8,and 2.6 times higher than were (NH4)2SO4,NH4NO3,and CON2H4,respectively.It was shown that yeast extract not only acted as a source of nitrogen,but also provided extra nutrition.A first-order kinetic model could be fitted to the oil degradation for the entire concentration range in optimum conditions.Furthermore,the half-life was found to be 26 h for oil 3 to 4 g · L-1.关键词
石油降解菌/假单胞菌/外加营养/降解动力学/酵母浸粉Key words
oil degradation bacteria/Pseudomons sp./extra nutrition/degradation kinetics/yeast extract分类
资源环境引用本文复制引用
于彩虹,赵粉红,吴东奎,丁燕燕,董聿森,朱晓晶..一株假单胞菌(Pseudomonas)SYBS01降解石油的特性[J].环境工程学报,2016,10(10):6042-6048,7.基金项目
国家高技术研究发展计划(863)项目(2007AA10Z404) （863）
教育部高校青年业务基金(2013QH11) （2013QH11）
大学生创新实验项目(201411413026) （201411413026）
