环境工程学报2016，Vol.10Issue(10)：6065-6070,6.DOI:10.12030/j.cjee.201505100
海洋红球菌SY095产生物表面活性剂发酵培养基的优化
Optimization fermentation medium for production of biosurfactant by Rhodococcus SY095
摘要
Abstract
To improve biosurfactant production,fermentation conditions of Rhodococcus SY095 were studied by optimization experiments.Based on the single factor experiment,soybean oil and urea were selected as the best carbon and nitrogen source,respectively.The Plackett-Burman design was used to evaluate the influence of related factors,and two significant factors,urea and monopotassium phosphate were selected.The path of steepest ascent and response surface methodology were used to explore optimal levels of significant factors.The optimal medium composition for biosurfactant production by Rhodococcus SY095 was determined as follows:soybean oil of 2％,urea of 2.35 g · L-1,monopotassium phosphate of 0.69 g · L-1,dipotassium hydrogen phosphate of 1 g · L-1,and magnesium sulfate 0.5 g · L-1.With this optimum medium,the surface tension of the culture could reach 29.054 mN · m-1,which was 12.9％ higher than that using the original medium.关键词
生物表面活性剂/发酵优化/Plackett-Burman设计/响应面分析Key words
Biosurfactant/fermentation optimization/Plackett-Burman design/response surface methodology分类
资源环境引用本文复制引用
张晓青,郝建安,杜瑾,姜天翔,王静,张雨山..海洋红球菌SY095产生物表面活性剂发酵培养基的优化[J].环境工程学报,2016,10(10):6065-6070,6.基金项目
海洋公益性行业科研专项项目(201305022) （201305022）
中央级公益性科研院所基本科研业务费专项资金资助项目(K-JBYWF-2015-G16,K-JBYWF-2015-T11,K-JBYWF-2016-T9) （K-JBYWF-2015-G16,K-JBYWF-2015-T11,K-JBYWF-2016-T9）
