黑龙江八一农垦大学学报2016，Vol.28Issue(5)：47-50,4.DOI:10.3969/j.issn.1002-2090.2016.05.009
2014~2015年黑龙江牡丹江地区犬博卡病毒的分子流行病学调查
Molecular Epidemiology Investigation of Canine Bocavirus in Mudanjiang Area of Heilongjiang Province from 2014 to 2015
摘要
Abstract
To investigate the evolution of CBoV strains circulating in Northeast China,40 fecal samples from rectal swabs of diarrheic dogs collected from May 2014 to April 2015 in Mudanjiang area. PCR amplification method was used to identify co-infection detec-tion and phylogenetic analysis. The PCR results indicated that 8 of the 40 fecal samples (20%)were positive for CBoV,co-infction of CPV-2,CCoV,and CaKV was 37.3%,25%,and 25%,respectively. Sequence analysis revealed that the partial NS1 genes of the 8 CBoV strains exhibited 94.2%~100% nucleotide identity,and 91.7%~100% amino acid identity. Phylogenetic analysis revealed that genetic diversity of the 8 CBoV strains from China compared with strains of CBoV from South Korea,the USA,Germany,and Hong Kong of China. The data revealed the presence of co-infection and genetic groups among the CBoV strains circulating in Mudanjiang area.关键词
犬博卡病毒/混合感染/遗传多样性Key words
canine bocaviruses/co-infection/genetic diversity分类
农业科技引用本文复制引用
姚爽,王志慧,滕井胜,李春秋,张思瑶,边秀东,张立春,郭东华..2014~2015年黑龙江牡丹江地区犬博卡病毒的分子流行病学调查[J].黑龙江八一农垦大学学报,2016,28(5):47-50,4.基金项目
黑龙江八一农垦大学研究生创新项目（YJSCX2015-Y28）。 （）
