核农学报2016，Vol.30Issue(12)：2289-2294,6.DOI:10.11869/j.issn.100-8551.2016.12.2289
浙贝母HMGR基因保守区序列的克隆及生物信息学分析
Cloning and Bioinformatics Analysis of Conserved Fragment of HMGR Gene in Fritillaria thunbergii
摘要
Abstract
HMGR is the first limit enzyme of mevalonate pathway,which play an important role in steroid alkaloid biosynthesis.In order to explore the regulation of HMGR in steroidal alkaloid syntheses in Fritillaria thunbergii,RT-PCR and TA cloning technology was used in this study and the first conserved fragment of HMGR gene in Fritillaria thunbergii,which was named FtHMGR,has been cloned.The total length of this fragment was 390bp.Sequence analysis showed that FtHMGR had high identity (79％ to 84％) to nine HMGR genes from various plants,the deduced amino acid sequence had a higher homology with other plants(95％ to 88％)and was closely related to that of Paris farqesii,Phoenix dectylifera and etc monocots.Cloning and molecular characterization of FtHMGR would be useful for further analysis of molecular regulation of steroid alkaloid biosynthesis in Fritillaria thunbergii.关键词
浙贝母/HMGR/基因克隆/生物信息学Key words
Fritillaria thunbergii/HMGR/cloning/bioinformatics引用本文复制引用
冯亚斌,俞信光,庄欣晨,王忠华..浙贝母HMGR基因保守区序列的克隆及生物信息学分析[J].核农学报,2016,30(12):2289-2294,6.基金项目
浙江省科技厅育种专项(2012C12912),宁波市公益类重大专项(2015C110007),浙江万里学院重中之重学科开放基金项目(KF2015001) （2012C12912）
