核农学报2016，Vol.30Issue(12)：2312-2317,6.DOI:10.11869/j.issn.100-8551.2016.12.2312
草菇V23子实体耐低温性能改良的初步研究
Preliminary Study on Improving the Tolerance of Volvariella volvacea Strain V23 Substance to Low-temperature
摘要
Abstract
To improve the performance of Volvariella volvacea strain V23 during low-temperature storage,The study performed mutagenesis on the protoplasts of V23 via electronic beam and60Co-γ ray radiation,respectively.Mutants with enhanced tolerance to low temperature were selected after three rounds of genome shuffling.One strain VF displaying normal fruiting and stable heredity was successfully obtained.The fruitbody of strain VF could be successfully stored for 20h at 10℃,which was 25％ longer than that of the original strain V23.The results proved that it is feasible to improve the low-temperature tolerance of Volvariella volvacea V23 using Genome shuffling technique.This work provides novel insights for cultivating edible fungi with enhanced performance under unfavorable environments.关键词
草菇/基因组重排技术/原生质体/耐低温菌株Key words
Volvariella volvacea/genome shuffling/protoplast/low temperature resistant strain引用本文复制引用
祝子坪,李娜,唐雪明..草菇V23子实体耐低温性能改良的初步研究[J].核农学报,2016,30(12):2312-2317,6.基金项目
国家自然科学青年基金(31300047) （31300047）
